Figure 4: Attenuation of H2O2-induced DNA damage by honokiol in HaCaT cells. HaCaT cells were treated with or without honokiol (10 |μM) or NAC (10 μM) for 1 h before treatment with H2O2 (500 μM) for 24 h. (A) Representative images of comet assay were presented (x400). (B) The DNA samples of cells were subjected to assessment of 8-OHdG level. The results are expressed as mean ± SD of triplicate determinations ( P < 0.05 compared with the control group; *P < 0.05 compared with the H2O2-treated group). (C) p-yH2AX and yH2AX expressions were identified by Western blotting analysis.