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  Indian J Med Microbiol
 

Figure 3: Inhibition of H2O2-induced ROS generation by honokiol in HaCaT cells. HaCaT cells were treated with 10 μM honokiol or 10 mM NAC for 1 h, and then stimulated with or without 500 μM H2O2 for another 1 h. (A) ROS production was measured using a flow cytometer. (B) The results are expressed as mean ± SD of triplicate determinations (*P < 0.05 compared with the control group; #P < 0.05 compared with the H2O2-treated group). (C) DCF fluorescence images of cells cultured under the same conditions were captured by a fluorescence microscope (×200).

Figure 3: Inhibition of H<sub>2</sub>O<sub>2</sub>-induced ROS generation by honokiol in HaCaT cells. HaCaT cells were treated with 10 μM honokiol or 10 mM NAC for 1 h, and then stimulated with or without 500 μM H<sub>2</sub>O<sub>2</sub> for another 1 h. (A) ROS production was measured using a flow cytometer. (B) The results are expressed as mean ± SD of triplicate determinations (*<i>P</i> < 0.05 compared with the control group; <sup>#</sup><i>P</i> < 0.05 compared with the H<sub>2</sub>O<sub>2</sub>-treated group). (C) DCF fluorescence images of cells cultured under the same conditions were captured by a fluorescence microscope (×200).