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  Indian J Med Microbiol
 

Figure 2: Suppression of H2O2-induced apoptosis by honokiol in HaCaT cells. HaCaT cells were pre-treated with or without honokiol (10 μM) or NAC (10 μM) for 1 h before treatment with H2O2 (500 μM) for 24 h. (A) After DAPI staining, the nuclei were observed using a fluorescence microscope (x400). (B) The percentages of apoptotic cells were determined by expressing the numbers of annexin V+ cells as percentages. The data are expressed as mean ± SD of three independent experiments (*P < 0.05 compared with the control group; *P < 0.05 compared with the H2O2-treated group).

Figure 2: Suppression of H<sub>2</sub>O<sub>2</sub>-induced apoptosis by honokiol in HaCaT cells. HaCaT cells were pre-treated with or without honokiol (10 μM) or NAC (10 μM) for 1 h before treatment with H<sub>2</sub>O<sub>2</sub> (500 μM) for 24 h. (A) After DAPI staining, the nuclei were observed using a fluorescence microscope (x400). (B) The percentages of apoptotic cells were determined by expressing the numbers of annexin V<sup>+</sup> cells as percentages. The data are expressed as mean ± SD of three independent experiments (*<i>P</i> < 0.05 compared with the control group; <i>*P</i> < 0.05 compared with the H<sub>2</sub>O<sub>2</sub>-treated group).