Figure 4: Protein expression of IKK-β (A-B), cyclin D1 (C-D) and Chk1 (E-F) in HT-29 and HCT 116 cells in different time points. Both cell lines were treated with maslinic acid at their IC50 for 12, 24, 48 and 72 h. The fold change of the protein expression levels in treated cells (maslinic acid) with respect to the vehicle control (DMSO) is shown in the line graphs against 4 different time points. Values represent mean ± SD of three independent experiments. *P < 0.05, **P < 0.01 with respect to the vehicle control group. IKK-β: inhibitor of κB kinase-β; Chk1: checkpoint kinase 1.