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  Indian J Med Microbiol
 

Figure 2: Real-time PCR results. Dilution series prepared from the plasmid encoding of Encephalitozoon intestinalis 16S SSU rRNA region were used as a positive control. The number of copies in the main stock dilution was calculated to be 2.8×108. Then, ten different dilutions were prepared by making a ten-fold dilution from the main stock.

Figure 2: Real-time PCR results. Dilution series prepared from the plasmid encoding of Encephalitozoon intestinalis 16S SSU rRNA region were used as a positive control. The number of copies in the main stock dilution was calculated to be 2.8×10<sup>8</sup>. Then, ten different dilutions were prepared by making a ten-fold dilution from the main stock.