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  Indian J Med Microbiol
 

Figure 4: Western blot results of p53 (A), Bax (B), Bcl-2 (C), mTOR (D), PTEN (E), Akt (F), and p38 MAPK (G) proteins in HT-29 cells treated with 5-FU, A. biebersteinii extracts, and combinations for 24 h. The results were normalized using protein expression rates of β-actin. 5-FU: 5-fluorouracil, ABH: A. biebersteinii hexane extract, ABC: A. biebersteinii chloroform extract, ABM: A. biebersteinii methanol extract. Compared with the control group, *: P<0.05, **: P<0.01, ***: P<0.001; compared with the 5-FU treated cells, #: P<0.05, ###: P<0.001.

Figure 4: Western blot results of p53 (A), Bax (B), Bcl-2 (C), mTOR (D), PTEN (E), Akt (F), and p38 MAPK (G) proteins in HT-29 cells treated with 5-FU, <i>A. biebersteinii</i> extracts, and combinations for 24 h. The results were normalized using protein expression rates of β-actin. 5-FU: 5-fluorouracil, ABH: <i>A. biebersteinii</i> hexane extract, ABC: <i>A. biebersteinii</i> chloroform extract, ABM: <i>A. biebersteinii</i> methanol extract. Compared with the control group, *: <i>P</i><0.05, **: <i>P</i><0.01, ***: <i>P</i><0.001; compared with the 5-FU treated cells, <sup>#</sup>: <i>P</i><0.05, <sup>###:</sup> <i>P</i><0.001.