Figure 4: Inhibitory effect of rice bran hydrolysates on THP-1 cell adhesion on LPS-stimulated HUVECs. HUVEC cells were seeded to form monolayer, incubated with rice bran hydrolysates and stimulated with LPS (100 ng/mL). THP-1 loaded with DCF-DA cells were overlaid on HUVECs. (A, B) Images and graph represent cell adhesion of THP-1 on HUVECs. (C, D) Cell lysates from HUVECs were analyzed for p-NF-κB and VCAM-1 by Western immunoblotting. Each bar represents mean ± SD of 3 experiments. *P < 0.05 vs. Control, #P <0.05 vs. LPS. HUVEC: human umbilical vein endothelial cells, LPS: lipopolysaccharide, DCF-DA: dichlorofluorescein diacetate, VCAM-1: vascular cell adhesion molecule-1.