Asian Pacific Journal of Tropical Biomedicine

ORIGINAL ARTICLE
Year
: 2021  |  Volume : 11  |  Issue : 2  |  Page : 74--80

Anti-senescence and anti-wrinkle activities of 3—bromo—4,5—dihydroxybenzaldehyde from Polysiphonia morrowii Harvey in human dermal fibroblasts


Su-Hyeon Cho1, Eun-Yi Ko2, Soo-Jin Heo3, Seo-Young Kim4, Juhee Ahn6, Kil-Nam Kim4 
1 Chuncheon Center, Korea Basic Science Institute; Department of Medical Biomaterials Engineering, College of Biomedical Sciences, Kangwon National University, Chuncheon 24341, Republic of Korea
2 Bio research Center, Dermapro, Jeju 63309, Republic of Korea
3 Jeju Marine Research Center, Korea Institute of Ocean Science & Technology, Jeju 63349, Republic of Korea
4 Chuncheon Center, Korea Basic Science Institute, Chuncheon 24341, Republic of Korea

Correspondence Address:
Juhee Ahn
Department of Medical Biomaterials Engineering, College of Biomedical Sciences, Kangwon National University, Chuncheon 24341
Republic of Korea
Kil-Nam Kim
Chuncheon Center, Korea Basic Science Institute, Chuncheon 24341
Republic of Korea

Objective: To investigate the anti-senescence effect of 3-bromo-4,5- dihydroxybenzaldehyde (BDB) from Polysiphonia morrowii Harvey in human dermal fibroblasts (HDF). Methods: HDF were subjected to treatment of BDB and then treated with hydrogen peroxide (H2O2) to induce premature senescence. Senescence-associated β-galactosidase (SA-β-gal) activity in HDF was determined using the SA-β-gal staining method. Intracellular reactive oxygen species (ROS) production was measured using the 2’,7’-dichlorodihydrofluorescein diacetate assay. Western blotting assay was performed to assess the level of antioxidant enzyme glutathione peroxidase 1 (GPX1). In addition, intracellular collagen and collagenase contents were analyzed using the respective ELISA kits. Elastase activity in HDF supernatants was measured from p-nitroaniline release and normalized using total protein content. Results: Treatment of HDF with H2O2 increased the activity of SAP-gal, but BDB pre-treatment resulted in the reduction of SA-β- gal activity. Moreover, BDB significantly reduced H2O2-induced intracellular ROS production. BDB also markedly increased the level of GPX1, which was inhibited by 400 µM of H2O2. Furthermore, in in vitro study, BDB significantly increased intracellular collagen content and decreased matrix metalloproteinase-1 and elastase activities in HDF. Conclusions: Our results demonstrate that BDB shows anti- senescence and anti-wrinkle activities in vitro.


How to cite this article:
Cho SH, Ko EY, Heo SJ, Kim SY, Ahn J, Kim KN. Anti-senescence and anti-wrinkle activities of 3—bromo—4,5—dihydroxybenzaldehyde from Polysiphonia morrowii Harvey in human dermal fibroblasts.Asian Pac J Trop Biomed 2021;11:74-80


How to cite this URL:
Cho SH, Ko EY, Heo SJ, Kim SY, Ahn J, Kim KN. Anti-senescence and anti-wrinkle activities of 3—bromo—4,5—dihydroxybenzaldehyde from Polysiphonia morrowii Harvey in human dermal fibroblasts. Asian Pac J Trop Biomed [serial online] 2021 [cited 2021 Jan 20 ];11:74-80
Available from: https://www.apjtb.org/article.asp?issn=2221-1691;year=2021;volume=11;issue=2;spage=74;epage=80;aulast=Cho;type=0