Asian Pacific Journal of Tropical Biomedicine

ORIGINAL ARTICLE
Year
: 2021  |  Volume : 11  |  Issue : 2  |  Page : 66--73

Corchorus olitorius aqueous extract attenuates quorum sensing-regulated virulence factor production and biofilm formation


Hanan M Al-Yousef1, Perwez Alam1, Zakia Khanam2, Musarat Amina1, Wafaa H. B. Hassan3 
1 Department of Pharmacognosy, College of Pharmacy, King Saud University, Riyadh, Saudi Arabia
2 University Malaysia Kelantan, Campus Jeli, 17600, Kelantan, Malaysia
3 Department of Pharmacognosy, Faculty of Pharmacy, Zagazig University, 44519 Zagazig, Egypt

Correspondence Address:
Hanan M Al-Yousef
Department of Pharmacognosy, College of Pharmacy, King Saud University, Riyadh
Saudi Arabia
Perwez Alam
Department of Pharmacognosy, College of Pharmacy, King Saud University, Riyadh
Saudi Arabia

Objective: To investigate the effect of Corchorus olitorius aqueous fraction (COAF) on quorum sensing (QS)-regulated virulence factors and biofilm formation in Pseudomonas aeruginosa (PAO1). Methods: The preliminary screening of the anti-QS effect of COAF was performed by evaluating the anti-pathogenic activity against Chromobacterium violaceum CV026 biosensor strain. Next, the inhibitory effects of COAF on QS-regulated pyocyanin production, proteolytic and elastolytic activities, swarming motility, and biofilm formation were evaluated in PAO1. Results: The results showed that the treatment of COAF significantly decreased the biofilm biomass, attenuated virulence factors, and inhibited swarming motility of PAO1 without affecting the growth of the bacteria in a dose-dependent manner. COAF at 2 000 μg/mL significantly decreased Las B elastase activity in PAO1 culture, exopolysaccharide production, swarming motility, pyocyanin level, and biomass of PAO1 by 55% (P<0.05), 60% (P<0.01), 61% (P<0.01), 65%(P<0.01) and 73% (P<0.01), respectively. In addition, the production of violacein was decreased by 62% (P<0.01) with the treatment of a high dose of COAF. Conclusions: These findings indicate that COAF can be a potential source of anti-QS agents.


How to cite this article:
Al-Yousef HM, Alam P, Khanam Z, Amina M, Hassan WH. Corchorus olitorius aqueous extract attenuates quorum sensing-regulated virulence factor production and biofilm formation.Asian Pac J Trop Biomed 2021;11:66-73


How to cite this URL:
Al-Yousef HM, Alam P, Khanam Z, Amina M, Hassan WH. Corchorus olitorius aqueous extract attenuates quorum sensing-regulated virulence factor production and biofilm formation. Asian Pac J Trop Biomed [serial online] 2021 [cited 2021 Jan 28 ];11:66-73
Available from: https://www.apjtb.org/article.asp?issn=2221-1691;year=2021;volume=11;issue=2;spage=66;epage=73;aulast=Al-Yousef;type=0