Asian Pacific Journal of Tropical Biomedicine

ORIGINAL ARTICLE
Year
: 2020  |  Volume : 10  |  Issue : 6  |  Page : 273--280

Standardized extract of Centella asiatica ECa 233 inhibits lipopolysaccharide-induced cytokine release in skin keratinocytes by suppressing ERK1/2 pathways


Furoida Moolsap1, Supita Tanasawet2, Mayuree H Tantisira3, Pilaiwanwadee Hutamekalin4, Varomyalin Tipmanee5, Wanida Sukketsiri1 
1 Department of Pharmacology, Faculty of Science, Prince of Songkla University, Songkhla, 90110, Thailand
2 Department of Anatomy, Faculty of Science, Prince of Songkla University, Songkhla, 90110, Thailand
3 Faculty of Pharmaceutical Science, Burapha University, Chon Buri Campus, Chon Buri, 20131, Thailand
4 Department of Physiology, Faculty of Science, Prince of Songkla University, Songkhla, 90110, Thailand
5 Department of Biomedical Sciences, Faculty of Medicine, Prince of Songkla University, Songkhla, 90110, Thailand

Correspondence Address:
Wanida Sukketsiri
Department of Pharmacology, Faculty of Science, Prince of Songkla University, Songkhla, 90110
Thailand

Objective: To evaluate the effect of standardized extract of Centella asiatica ECa 233 on inflammatory mediator production through cyclooxygenase-2 (COX-2), extracellular signal-regulated kinase 1/2 (ERK1/2) and nuclear factor-κB (NF-κB) pathway in keratinocyte HaCaT cells. Methods: HaCaT cells were treated with 0.1, 1, 10 and 100 μg/mL ECa 233 in the presence of lipopolysaccharide (LPS). Proinflammatory cytokines and prostaglandin E2 were assessed with ELISA. Western blotting was used to determine the inhibition of COX-2, ERK1/2 and NF-κB protein expression. Results: ECa 233 suppressed LPS-induced release of interleukin- 1β, tumor necrosis factor-α, and prostaglandin E2. ECa 233 also inhibitedCOX-2, phosphorylation of ERK1/2 and the activation of NF-κB. Moreover, the formation of reactive oxygen species (ROS) was decreased in response to LPS-inflamed keratinocytes. Conclusions: ECa 233 inhibits LPS-stimulated production of inflammatory mediators in keratinocytes via suppressing ERK1/2 andNF-κB pathways. The suppressive effect of ECa 233 may be related to an inhibition of ROS production.


How to cite this article:
Moolsap F, Tanasawet S, Tantisira MH, Hutamekalin P, Tipmanee V, Sukketsiri W. Standardized extract of Centella asiatica ECa 233 inhibits lipopolysaccharide-induced cytokine release in skin keratinocytes by suppressing ERK1/2 pathways.Asian Pac J Trop Biomed 2020;10:273-280


How to cite this URL:
Moolsap F, Tanasawet S, Tantisira MH, Hutamekalin P, Tipmanee V, Sukketsiri W. Standardized extract of Centella asiatica ECa 233 inhibits lipopolysaccharide-induced cytokine release in skin keratinocytes by suppressing ERK1/2 pathways. Asian Pac J Trop Biomed [serial online] 2020 [cited 2021 Jan 17 ];10:273-280
Available from: https://www.apjtb.org/article.asp?issn=2221-1691;year=2020;volume=10;issue=6;spage=273;epage=280;aulast=Moolsap;type=0