Asian Pacific Journal of Tropical Biomedicine

ORIGINAL ARTICLE
Year
: 2020  |  Volume : 10  |  Issue : 11  |  Page : 505--515

Achillea biebersteinii extracts suppress angiogenesis and enhance sensitivity to 5-fluorouracil of human colon cancer cells via the PTEN/AKT/mTOR pathway in vitro


Mehmet Kadir Erdogan1, Can Ali Ağca2, Hakan Aşkın3 
1 Department of Biology, Faculty of Arts and Sciences, Bingol University, 12000, Bingol, Turkey
2 Department of Molecular Biology and Genetics, Faculty of Arts and Sciences, Bingol University, 12000, Bingol, Turkey
3 Department of Molecular Biology and Genetics, Faculty of Sciences, Ataturk University, 25000, Erzurum, Turkey

Correspondence Address:
Mehmet Kadir Erdogan
Department of Biology, Faculty of Arts and Sciences, Bingol University, 12000, Bingol
Turkey

Objective: To investigate the antiproliferative, anti-angiogenic, and apoptotic effects of extracts of Achillea biebersteinii (ABE) and combined treatments of ABE with 5-fluorouracil (5-FU) on HT-29 cells. Methods: The effects of ABE, 5-FU, and combined treatments on the viability of HT-29 cells were determined by 3-(4,5- dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. Isobologram analysis was used to determine synergism between ABE and 5-FU. The apoptotic and anti-angiogenic effects were determined by cell death detection and human vascular endothelial growth factor ELISA method, respectively. Transcriptional and translational expressions of p53, Bax, Bcl-2, p38 MAPK, Akt, PTEN, and mTOR were also evaluated by real-time PCR and Western blotting analysis. Results: ABE decreased the viability of HT-29 cells in a dose-dependent manner. Combined treatment of hexane, chloroform, and methanol extracts of Achillea biebersteinii with 5-FU at IC50 doses decreased the cell viability to 26.0%, 19.1%, and 14.9%, respectively (P<0.001). Furthermore, ABE treatment alone and combination with 5-FU, induced apoptosis, significantly downregulated mTOR, Akt, Bcl-2 expression, upregulated p53, Bax, PTEN, p38 MAPK expression, and exhibited anti-angiogenetic effects. Conclusions: Our findings indicate that ABE shows synergism with 5-FU and inhibits the proliferation of HT-29 cells by inducing apoptosis and suppressing angiogenesis, which may provide biological evidence for further use of ABE in the treatment of colorectal cancer.


How to cite this article:
Erdogan MK, Ağca CA, Aşkın H. Achillea biebersteinii extracts suppress angiogenesis and enhance sensitivity to 5-fluorouracil of human colon cancer cells via the PTEN/AKT/mTOR pathway in vitro.Asian Pac J Trop Biomed 2020;10:505-515


How to cite this URL:
Erdogan MK, Ağca CA, Aşkın H. Achillea biebersteinii extracts suppress angiogenesis and enhance sensitivity to 5-fluorouracil of human colon cancer cells via the PTEN/AKT/mTOR pathway in vitro. Asian Pac J Trop Biomed [serial online] 2020 [cited 2020 Dec 2 ];10:505-515
Available from: https://www.apjtb.org/article.asp?issn=2221-1691;year=2020;volume=10;issue=11;spage=505;epage=515;aulast=Erdogan;type=0