Asian Pacific Journal of Tropical Biomedicine

ORIGINAL ARTICLE
Year
: 2020  |  Volume : 10  |  Issue : 10  |  Page : 460--469

Anti-MMP-2 and MMP-9 activity of Salsola komarovii Iljin extract and its solvent fractions


Jung-Ha Kil1, Fatih Karadeniz1, Ga Hyun Yu2, Hojun Kim3, Junse Kim3, Jung Hwan Oh1, Jung Im Lee1, Chang-Suk Kong4, Youngwan Seo3 
1 Marine Biotechnology Center for Pharmaceuticals and Foods, Silla University, Baegyang-daero 700 beon-gil 140, Sasang-gu, Busan 46958, Korea
2 Department of Food and Nutrition, College of Medical and Life Sciences, Silla University, Baegyang-daero 700 beon-gil 140, Sasang-gu, Busan 46958, Korea
3 Division of Marine Bioscience, Korea Maritime and Ocean University, Busan 49112, Korea
4 Marine Biotechnology Center for Pharmaceuticals and Foods; Department of Food and Nutrition, College of Medical and Life Sciences, Silla University, Baegyang-daero 700 beon-gil 140, Sasang-gu, Busan 46958, Korea

Correspondence Address:
Youngwan Seo
Division of Marine Bioscience, Korea Maritime and Ocean University, Busan 49112
Korea

Objective: To investigate matrix metalloproteinases (MMP)-2 and MMP-9 inhibitory effect of Salsola komarovii Iljin, an edible halophyte with health beneficial effects. Methods: Salsola komarovii crude extracts (SKI), and solvent (n-hexane, 85% aq. MeOH, n-BuOH, and H2O) fractionated extracts of SKI were prepared. Gelatin zymography was carried out to observe MMP enzymatic activity. The release of the MMP enzymes was measured by enzyme-linked immunosorbent assay. Expression of MMPs in mRNA and protein level were investigated by polymerase chain reaction analysis and immunoblotting, respectively. Results: SKI and SKI fractions inhibited active MMP-2 and MMP-9 amount in the treated cell culture medium. Also, SKI suppressed the release of MMP-2 and MMP-9 from stimulated HT1080 human fibrosarcoma cells. Furthermore, SKI suppressed the mRNA and protein expression of MMP-2 and MMP-9. SKI fractions showed parallel effects except for H2O fraction which did not yield any significant MMP inhibitory effect. Among fractions, 85% aq. MeOH was the most active fraction to inhibit both the enzymatic effect and expression of MMP-2 and MMP-9. Conclusions: SKI may contain potential MMP release inhibitory compounds. Salsola komarovii is a promising source of compounds against MMP and could be utilized in the development of antitumor agents.


How to cite this article:
Kil JH, Karadeniz F, Yu GH, Kim H, Kim J, Oh JH, Lee JI, Kong CS, Seo Y. Anti-MMP-2 and MMP-9 activity of Salsola komarovii Iljin extract and its solvent fractions.Asian Pac J Trop Biomed 2020;10:460-469


How to cite this URL:
Kil JH, Karadeniz F, Yu GH, Kim H, Kim J, Oh JH, Lee JI, Kong CS, Seo Y. Anti-MMP-2 and MMP-9 activity of Salsola komarovii Iljin extract and its solvent fractions. Asian Pac J Trop Biomed [serial online] 2020 [cited 2021 Jan 18 ];10:460-469
Available from: https://www.apjtb.org/article.asp?issn=2221-1691;year=2020;volume=10;issue=10;spage=460;epage=469;aulast=Kil;type=0