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Anti-senescence and anti-wrinkle activities of 3—bromo—4,5—dihydroxybenzaldehyde from Polysiphonia morrowii Harvey in human dermal fibroblasts
Su-Hyeon Cho1, Eun-Yi Ko2, Soo-Jin Heo3, Seo-Young Kim4, Juhee Ahn5, Kil-Nam Kim4
1 Chuncheon Center, Korea Basic Science Institute; Department of Medical Biomaterials Engineering, College of Biomedical Sciences, Kangwon National University, Chuncheon 24341, Republic of Korea
2 Bio research Center, Dermapro, Jeju 63309, Republic of Korea
3 Jeju Marine Research Center, Korea Institute of Ocean Science & Technology, Jeju 63349, Republic of Korea
4 Chuncheon Center, Korea Basic Science Institute, Chuncheon 24341, Republic of Korea
5 Department of Medical Biomaterials Engineering, College of Biomedical Sciences, Kangwon National University, Chuncheon 24341, Republic of Korea
Correspondence Address:
Juhee Ahn
Department of Medical Biomaterials Engineering, College of Biomedical Sciences, Kangwon National University, Chuncheon 24341
Republic of Korea
Kil-Nam Kim
Chuncheon Center, Korea Basic Science Institute, Chuncheon 24341
Republic of Korea
 Source of Support: This research was supported by Korea Basic Science Institute (grant number C39260), National Research Foundation of Korea (NRF) funded by the Korea government (MSIT) (grant number: NRF- 2019R1C1C1005608), and a research grant from the Korea Institute of Ocean Science and Technology (PE99822), Conflict of Interest: None
DOI: 10.4103/2221-1691.303606
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Objective: To investigate the anti-senescence effect of 3-bromo-4,5- dihydroxybenzaldehyde (BDB) from Polysiphonia morrowii Harvey in human dermal fibroblasts (HDF).
Methods: HDF were subjected to treatment of BDB and then treated with hydrogen peroxide (H2O2) to induce premature senescence. Senescence-associated β-galactosidase (SA-β-gal) activity in HDF was determined using the SA-β-gal staining method. Intracellular reactive oxygen species (ROS) production was measured using the 2’,7’-dichlorodihydrofluorescein diacetate assay. Western blotting assay was performed to assess the level of antioxidant enzyme glutathione peroxidase 1 (GPX1). In addition, intracellular collagen and collagenase contents were analyzed using the respective ELISA kits. Elastase activity in HDF supernatants was measured from p-nitroaniline release and normalized using total protein content.
Results: Treatment of HDF with H2O2 increased the activity of SAP-gal, but BDB pre-treatment resulted in the reduction of SA-β- gal activity. Moreover, BDB significantly reduced H2O2-induced intracellular ROS production. BDB also markedly increased the level of GPX1, which was inhibited by 400 µM of H2O2. Furthermore, in in vitro study, BDB significantly increased intracellular collagen content and decreased matrix metalloproteinase-1 and elastase activities in HDF.
Conclusions: Our results demonstrate that BDB shows anti- senescence and anti-wrinkle activities in vitro. |
