Impact Factor 2019 : 1.903 (@Clarivate Analytics)
  • Users Online: 259
  • Print this page
  • Email this page
ORIGINAL ARTICLE
Year : 2020  |  Volume : 10  |  Issue : 9  |  Page : 417-425

In vitro antioxidant and anti-cancer activities and phytochemical analysis of Commelina benghalensis L. root extracts


1 University Institute of Biochemistry and Biotechnology, PMAS-UAAR, Rawalpindi, Pakistan
2 Department of Botany, GDC Khanpur, Haripur, Pakistan
3 Department of Plant Sciences, Faculty of Biological Sciences, Quaid-i-Azam University, Islamabad- 45320, Pakistan
4 Department of Zoology, University of Okara, Okara, Pakistan
5 Department of Pharmacology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore
6 Department of Biotechnology, Faculty of Biological Sciences, Quaid-i-Azam University, Islamabad- 45320, Pakistan

Correspondence Address:
Tariq Mahmood
Department of Plant Sciences, Faculty of Biological Sciences, Quaid-i-Azam University, Islamabad- 45320
Pakistan
Login to access the Email id

Source of Support: None, Conflict of Interest: None


DOI: 10.4103/2221-1691.290133

Get Permissions

Objective: To explore antioxidant potential, anti-cancer activity, and phytochemicals of Commelina benghalensis L. Methods: The roots of Commelina benghalensis were extracted in different solvents (methanol, ethanol, benzene, chloroform, n-hexane) with a range of polarity. Antioxidant activity was evaluated by reducing power assay, DPPH radical scavenging activity and phosphomolybdenum method, cytotoxicity by MTT assay, apoptotic and cell cycle analysis by flow cytometry, migratory and invasive potential by wound scratch assay and invasion assay, respectively, functional groups analysis by FT-IR spectroscopy and phytochemicals by aluminum chloride colorimetric and Folin- Ciocalteu methods. Results: The extracts showed worthy antioxidant potential. The chloroform extract demonstrated the most significant cytotoxic effect on MDA-MB-231 (breast cancer) cell line, induced apoptosis and reduced migratory and invasive potential of MDA-MB-231 cells. Methanol and ethanol extracts presented good yield of total phenolic and total flavonoid contents. The FTIR spectroscopic studies revealed different characteristic peak values with various functional compounds such as alkenes, alkanes, aliphatic amines, aromatics, alkyl halides, carboxylic acid, alcohols, ester, aldehydes and ketones. Conclusions: The results demonstrate the potential use of Commelina benghalensis as a good antioxidant with significant anti-cancer effect.


[FULL TEXT] [PDF]*
Print this article     Email this article
 Next article
 Previous article
 Table of Contents

 Similar in PUBMED
   Search Pubmed for
   Search in Google Scholar for
 Related articles
 Citation Manager
 Access Statistics
 Reader Comments
 Email Alert *
 Add to My List *
 * Requires registration (Free)
 

 Article Access Statistics
    Viewed359    
    Printed15    
    Emailed0    
    PDF Downloaded169    
    Comments [Add]    

Recommend this journal