Enzyme-treated date plum leave extract ameliorates atopic dermatitis-like skin lesion in hairless mice
Byoung Ok Cho1, Jae Young Shin2, Ji-Su Kim3, Denis Nchang Che4, Hyun Ju Kang2, Hyeon Jin Kang5, Hyeonhwa Oh5, Young-Soo Kim5, Seon Il Jang1
1 Research Institute, Ato Q&A Co. Ltd, Jeonju-si, Jeonbuk 54840; Department of Health Management, Jeonju University, Jeonju-si, Jeonbuk 55069, Republic of Korea
2 Research Institute, Ato Q&A Co. Ltd, Jeonju-si, Jeonbuk 54840, Republic of Korea
3 Department of Health Management; Agro-Bio and Food Industry, Jeonju University, Jeonju-si, Jeonbuk 55069, Republic of Korea
4 Department of Health Management, Jeonju University, Jeonju-si, Jeonbuk 55069; Department of Food Science and Technology, Chonbuk National University, Jeonju-si, Jeonbuk 54896, Republic of Korea
5 Department of Food Science and Technology, Chonbuk National University, Jeonju-si, Jeonbuk 54896, Republic of Korea
Seon Il Jang
Research Institute, Ato Q&A Co. Ltd, Jeonju-si, Jeonbuk 54840; Department of Health Management, Jeonju University, Jeonju-si, Jeonbuk 55069
Republic of Korea
Source of Support: This research was financially supported by the Ministry of Small
and Medium Enterprise and Startups (MSS), Korea, under the
“Regional Specialized Industry Development Program (Project
number P0002904)” supervised by the Korea Institute for
Advancement of Technology, Conflict of Interest: None
Objective: To evaluate the effect of different extracts of Diospyros lotus leaves in atopic dermatitis
Methods: Diospyros lotus leaves were extracted in ethanol and treated with or without hydrochloric acid or α-rhamnosidase to obtain three different extracts-ethanol, acid-hydrolyzed, and enzyme-hydrolyzed leaf extracts of date plum. The myricitrin content in all samples was measured using HPLC analysis. In vitro antioxidant and anti-inflammatory activities of the extracts were determined by measuring DPPH radical scavenging activities and nitric oxide production in RAW264.7 cells, respectively. Seven- week-old male hairless mice were used to evaluate the anti-atopic dermatitis effects of three extracts in vivo. Splenocytes and mast cells were used to further determine the anti-atopic dermatitis effects of the major compound in the ethanol leaf extract.
Results: Enzyme-hydrolyzed leaf extract showed significant in vitro antioxidant and anti-inflammatory activities, and attenuated atopic dermatitis-like skin symptoms and clinical signs more significantly than ethanol and acid-hydrolyzed leaf extracts in 1-fluoro-2,4- dinitrobenzene and house dust mite antigen-treated hairless mice. Enzyme-hydrolyzed leaf extract also suppressed the serum level of immunoglobulin E, interleukin (IL)-4, tumor necrosis factor (TNF)-α, interferon (IFN)-γ, thymic stromal lymphopoietin, and thymus and activation-regulated chemokine in mice with atopic dermatitis. Furthermore, histological analysis revealed that enzyme- hydrolyzed leaf extract suppressed the increased epidermal thickness, dermal infiltration of inflammatory cells, and infiltration and degranulation of mast cells more markedly than the other two extracts in atopic dermatitis-like skin lesions. In addition, this extract effectively inhibited the production of IFN-γ, IL-4,and thymus and activation-regulated chemokine compared with the other two extracts in concanavalin A-stimulated splenocytes. Myricitrin, a major compound of enzyme-hydrolyzed leaf extract, suppressed atopic dermatitis biomarkers in stimulated mouse splenocytes and HMC-1 human mast cells.
Conclusions: These results suggest that enzyme-hydrolyzed leaf extract might be a potential candidate to treat atopic dermatitis.