Crebanine N-oxide, a natural aporphine alkaloid isolated from Stephania hainanensis, induces apoptosis and autophagy in human gastric cancer SGC-7901 cells
Zheng-Wen Wang1, Hao Liu2, Geng-Tai Ye2, Zhi-Yong Sheng2, Yan-Feng Hu2, Yin-Feng Tan3, Guo-Xin Li2
1 Department of General Surgery, Nanfang Hospital, Southern Medical University, Guangzhou 510515, Guangdong; Department of Hepatopancreatobiliary Surgery and General Surgery, Hainan Cancer Hospital, Affiliated Cancer Hospital of Hainan Medical University, Haikou 570312, Hainan, China
2 Department of General Surgery, Nanfang Hospital, Southern Medical University, Guangzhou 510515, Guangdong, China
3 Hainan Provincial Key Laboratory of R&D on Tropical Herbs, Hainan Medical University, Haikou 571199, Hainan, China
Department of General Surgery, Nanfang Hospital, Southern Medical University, Guangzhou 510515, Guangdong
Source of Support: This research was supported by the National Natural Science
Foundation of China (NO.81760628), Conflict of Interest: None
Objective: To investigate the cytotoxic effects and the potential mechanisms of crebanine N-oxide in SGC-7901 gastric adenocarcinoma cells.
Methods: The cytotoxicity of crebanine N-oxide was evaluated by 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide assay and cellular morphology was observed under a microscope. Cell apoptosis was determined by flow cytometry using propidium iodide staining. The expression levels of apoptotic-related proteins, cleaved caspase-3, cytochrome C, p53 and Bax, and autophagy- related proteins p62, beclin1 and LC3 were detected by Western blotting assays.
Results: Crebanine N-oxide treatment significantly inhibited the proliferation of SGC-7901 cells in a dose-dependent and time- dependent manner via induction of G2-phase cell cycle arrest, apoptosis, and autophagy in SGC-7901 cells.
Conclusions: Crebanine N-oxide could inhibit the growth of gastric cancer cells by promoting apoptosis and autophagy and could be used as a potential agent for treating gastric cancer.