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ORIGINAL ARTICLE
Year : 2020  |  Volume : 10  |  Issue : 5  |  Page : 224-231

Crebanine N-oxide, a natural aporphine alkaloid isolated from Stephania hainanensis, induces apoptosis and autophagy in human gastric cancer SGC-7901 cells


1 Department of General Surgery, Nanfang Hospital, Southern Medical University, Guangzhou 510515, Guangdong; Department of Hepatopancreatobiliary Surgery and General Surgery, Hainan Cancer Hospital, Affiliated Cancer Hospital of Hainan Medical University, Haikou 570312, Hainan, China
2 Department of General Surgery, Nanfang Hospital, Southern Medical University, Guangzhou 510515, Guangdong, China
3 Hainan Provincial Key Laboratory of R&D on Tropical Herbs, Hainan Medical University, Haikou 571199, Hainan, China

Correspondence Address:
Guo-Xin Li
Department of General Surgery, Nanfang Hospital, Southern Medical University, Guangzhou 510515, Guangdong
China
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Source of Support: This research was supported by the National Natural Science Foundation of China (NO.81760628), Conflict of Interest: None


DOI: 10.4103/2221-1691.281466

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Objective: To investigate the cytotoxic effects and the potential mechanisms of crebanine N-oxide in SGC-7901 gastric adenocarcinoma cells. Methods: The cytotoxicity of crebanine N-oxide was evaluated by 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide assay and cellular morphology was observed under a microscope. Cell apoptosis was determined by flow cytometry using propidium iodide staining. The expression levels of apoptotic-related proteins, cleaved caspase-3, cytochrome C, p53 and Bax, and autophagy- related proteins p62, beclin1 and LC3 were detected by Western blotting assays. Results: Crebanine N-oxide treatment significantly inhibited the proliferation of SGC-7901 cells in a dose-dependent and time- dependent manner via induction of G2-phase cell cycle arrest, apoptosis, and autophagy in SGC-7901 cells. Conclusions: Crebanine N-oxide could inhibit the growth of gastric cancer cells by promoting apoptosis and autophagy and could be used as a potential agent for treating gastric cancer.


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