Anti-MMP-2 and MMP-9 activity of Salsola komarovii Iljin extract and its solvent fractions
Jung-Ha Kil1, Fatih Karadeniz1, Ga Hyun Yu2, Hojun Kim3, Junse Kim3, Jung Hwan Oh1, Jung Im Lee1, Chang-Suk Kong4, Youngwan Seo3
1 Marine Biotechnology Center for Pharmaceuticals and Foods, Silla University, Baegyang-daero 700 beon-gil 140, Sasang-gu, Busan 46958, Korea
2 Department of Food and Nutrition, College of Medical and Life Sciences, Silla University, Baegyang-daero 700 beon-gil 140, Sasang-gu, Busan 46958, Korea
3 Division of Marine Bioscience, Korea Maritime and Ocean University, Busan 49112, Korea
4 Marine Biotechnology Center for Pharmaceuticals and Foods; Department of Food and Nutrition, College of Medical and Life Sciences, Silla University, Baegyang-daero 700 beon-gil 140, Sasang-gu, Busan 46958, Korea
Division of Marine Bioscience, Korea Maritime and Ocean University, Busan 49112
Source of Support: This research was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (No. 2016R1D1A1B03932769) and by the Ministry of Science and ICT (No. 2019R1F1A1059325), Conflict of Interest: None
Objective: To investigate matrix metalloproteinases (MMP)-2 and MMP-9 inhibitory effect of Salsola komarovii Iljin, an edible halophyte with health beneficial effects.
Methods: Salsola komarovii crude extracts (SKI), and solvent (n-hexane, 85% aq. MeOH, n-BuOH, and H2O) fractionated extracts of SKI were prepared. Gelatin zymography was carried out to observe MMP enzymatic activity. The release of the MMP enzymes was measured by enzyme-linked immunosorbent assay. Expression of MMPs in mRNA and protein level were investigated by polymerase chain reaction analysis and immunoblotting, respectively.
Results: SKI and SKI fractions inhibited active MMP-2 and MMP-9 amount in the treated cell culture medium. Also, SKI suppressed the release of MMP-2 and MMP-9 from stimulated HT1080 human fibrosarcoma cells. Furthermore, SKI suppressed the mRNA and protein expression of MMP-2 and MMP-9. SKI fractions showed parallel effects except for H2O fraction which did not yield any significant MMP inhibitory effect. Among fractions, 85% aq. MeOH was the most active fraction to inhibit both the enzymatic effect and expression of MMP-2 and MMP-9.
Conclusions: SKI may contain potential MMP release inhibitory compounds. Salsola komarovii is a promising source of compounds against MMP and could be utilized in the development of antitumor agents.