Mucus from different fish species alleviates carrageenan-induced inflammatory paw edema in rats
Mustafa Hitit1, Orhan Corum2, Mehmet Ozbek3, Kamil Uney4, Ertugrul Terzi5, Gokhan Arslan6, AY Sonmez5
1 Department of Genetics, Faculty of Veterinary Medicine, Kastamonu University, Kastamonu, 37200, Turkey
2 Department of Pharmacology and Toxicology, Faculty of Veterinary Medicine, Kastamonu University, Kastamonu, 37200, Turkey
3 Department of Histology and Embryology, Faculty of Veterinary Medicine, Mehmet Akif Ersoy University, Burdur, 15030, Turkey
4 Department of Pharmacology and Toxicology, Faculty of Veterinary Medicine, Selcuk University, Konya, 42031, Turkey
5 Faculty of Fisheries, Kastamonu University, Kastamonu, 37200, Turkey
6 Faculty of Fisheries, Ataturk University, Erzurum, 25240, Turkey
Department of Genetics, Faculty of Veterinary Medicine, Kastamonu University, Kastamonu, 37200
Source of Support: This study was supported by the Coordination Unit of Scientific
Research Projects, University of Kastamonu (Project No. KUBAP01/
2018-72), Conflict of Interest: None
Objective: To determine the anti-inflammatory effects of mucus obtained from different fish species on the carrageenan-induced acute paw edema in rats.
Methods: Forty-two rats were randomly divided into seven groups. Acute paw edema was induced by 0.1 mL of 1% carrageenan, and a single dose of diclofenac and lyophilized mucus (25 mg/kg) of rainbow trout, brook trout, European sea bass, and gilthead sea bream were administered to rats through gastric gavage 1 h before carrageenan treatment. Rat paws were measured before and 1-4 h after carrageenan treatment. The mRNA expressions of cytokines (TNF-α, IL-1β, IL-6, IL-10, and TGF-β), antioxidant markers (catalase and superoxide dismutase), and COX-2 were investigated using quantitative polymerase chain reaction. The histopathological changes were evaluated by hematoxylin and eosin staining.
Results: The inhibition percentage of carrageenan-induced paw edema by different fish mucus ranged from 52.46% to 74.86% at 4 h. Histopathological evaluation showed that all fish mucus diminished carrageenan-induced edema and inflammatory cell infiltration. The upregulation of IL-1β mRNA induced by carrageenan was decreased by the mucus of rainbow trout and gilthead sea bream while an increase in the expression of IL-6 mRNA was reduced by the mucus of rainbow trout, brook trout, and gilthead sea bream. In addition, the mRNA expression of superoxide dismutase was higher in the rainbow trout mucus group than the carrageenan group.
Conclusions: Mucus obtained from different fish species may have anti-inflammatory effects.