Antidiarrheal potential of Distemonanthus benthamianus Baillon. extracts via inhibiting voltage-dependent calcium channels and cholinergic receptors
William Yousseu Nana1, Gilbert Ateufack2, Marius Mbiantcha2, Shamim Khan3, Hafiz Majid Rasheed3, Albert Atsamo4, Abdul Jabbar Shah3, Albert Kamanyi2, Taous Khan3
1 Research Unit of Animal Physiology and Phytopharmacology, Faculty of Science, University of Dschang, PO. Box 67 Dschang, Cameroon; Department of Pharmacy, COMSATS University Islamabad, Abbottabad Campus 22060, Pakistan
2 Research Unit of Animal Physiology and Phytopharmacology, Faculty of Science, University of Dschang, PO. Box 67 Dschang, Cameroon
3 Department of Pharmacy, COMSATS University, Islamabad, Abbottabad Campus 22060, Pakistan
4 Laboratory of Animal Physiology, Faculty of Science, University of Yaoundé 1, PO. Box 812 Yaoundé, Cameroon
Research Unit of Animal Physiology and Phytopharmacology, Faculty of Science, University of Dschang, PO. Box 67 Dschang
Source of Support: This work was supported by the CIIT-TWAS Sandwich Postgraduate
Fellowship (FR number: 3240293217, 2016), Conflict of Interest: None
Objective: To evaluate spasmolytic mechanisms of aqueous and methanolic extracts from Distemonanthus benthamianus trunk-bark.
Methods: Spasmolytic activities of extracts were evaluated in vitro on spontaneous and potassium chloride-induced jejunum contractions, or against cholinergic [acetylcholine (0.3 μmol/L)] stimulations. High performance liquid chromatography analysis of both extracts was performed in reference to standard compounds.
Results: Extracts developed concentration-dependent inhibitory activities. The methanolic extract, which revealed better activity, produced spasmolytic and myorelaxant effects at concentrations of 0.01-0.30 mg/mL with EC50 of 0.06 and 0.09 mg/mL (95% CI: 0.03-0.3 mg/mL), respectively. Its anticholinergic effect was obtained at the same concentrations with EC50 of 0.11 mg/mL (95% CI: 0.03-0.3 mg/mL). Chromatograms showed the presence of gallic acid in both extracts, rutin being only detected in the aqueous extract.
Conclusions: Distemonanthus benthamianus extracts exhibit verapamil and atropine-like activities, thus highlighting calcium channels and muscarinic receptors blocking potentials, which may be conveyed by some phenolic compounds. These results confirm the antidiarrheal activity of Distemonanthus benthamianus extracts.