| ANTIMALARIAL RESEARCH |
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| Year : 2018 | Volume
: 8
| Issue : 5 | Page : 268-272 |
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Antiplasmodial activity of silver nanoparticles: A novel green synthesis approach
Madhur Sardana1, Varnika Agarwal1, Akansha Pant2, Vinita Kapoor1, Kailash C Pandey3, Sanjay Kumar1
1 Department of Chemistry, Sri Venkateswara College, University of Delhi, Delhi 110 021, India
2 National Institute of Malaria Research, ICMR, New Delhi-110077, India
3 Department of Biochemistry, National Institute for Research in Environmental Health, ICMR, Bhopal, India
Correspondence Address:
Sanjay Kumar
Department of Chemistry, Sri Venkateswara College, University of Delhi, Delhi 110 021
India
 Source of Support: None, Conflict of Interest: None  | 5 |
DOI: 10.4103/2221-1691.233008
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Objective: To synthesize silver nanoparticles using silver nitrate by a green technique which involves different compositions of aqueous leaf extracts of Azadirachta indica (neem) and Ocimum sanctum (tulsi). Methods: Their shape and size were determined using transmission electron microscopy and UV-visible spectroscopy. Their antiplasmodial activity was studied using the malarial parasite strain (Plasmodium falciparum, 3D7). The parasite strain (3D7) was collected and revived in vitro using Trager and Jensen method in RPMI 1640 medium for 7-8 cycles. Half maximal effective concentration values were calculated by nonlinear regression analysis. Results: Transmission electron microscopy results confirmed the formation of silver nanoparticles with size ranging from 4.74-39.32 nm and their size differs by varying the concentrations from 20% to 100% of neem extract in neem and tulsi extracts. It was observed that samples B and C showed half maximum effective concentration of about 0.3 μ M. Conclusions: It can be easily established that the aqueous leaf extracts of neem and tulsi in combination can be a good source for synthesis of silver nanoparticles with small size possessing appreciable antiplasmodial activity.
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