| BASIC RESEARCH |
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| Year : 2018 | Volume
: 8
| Issue : 5 | Page : 245-253 |
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Phytochemical bioprospecting, antioxidant, antimicrobial and cytotoxicity activities of saline extract from Tithonia diversifolia (Hemsl) A. Gray leaves
Bruno Rafael Barboza1, Bárbara Rafaela da Silva Barros1, Bárbara de Azevedo Ramos2, Maiara Celine de Moura2, Thiago Henrique Napoleão2, Maria Tereza dos Santos Correia2, Luana Cassandra Breitenbach Barroso Coelho2, Iranildo José da Cruz Filho1, Ana Maria Souto Maior1, Túlio Diego da Silva3, Leylianne de Cássia Rodrigues Nerys4, Edson Renan Barros de Santana4, Cláudia Sampaio de Andrade Lima4, Virgínia Maria Barros de Lorena5, Cristiane Moutinho Lagos de Melo1
1 Department of Antibiotics, Biosciences Centre, Federal University of Pernambuco, Pernambuco, Brazil
2 Department of Biochemistry, Biosciences Centre, Federal University of Pernambuco, Pernambuco, Brazil
3 Center for Strategic Technologies of the Northeast, Pernambuco, Brazil
4 Department of Biophysics, Biosciences Centre, Federal University of Pernambuco, Pernambuco, Brazil
5 Aggeu Magalhães Institute, IAM/FIOCRUZ, Pernambuco, Brazil
Correspondence Address:
Cristiane Moutinho Lagos de Melo
Departamento de Antibióticos, Universidade Federal de Pernambuco, Pernambuco
Brazil
 Source of Support: None, Conflict of Interest: None  | 3 |
DOI: 10.4103/2221-1691.233005
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Objective: To evaluate antimicrobial and antioxidant properties of saline extract from Tithonia diversifolia leaves by phytochemical bioprospecting, and investigate its safety against animal cells. Methods: The saline extract was prepared, with NaCl (0.15 M), by constant stirring of the dried and pulverized leaves, followed by volume reduction by lyophilization. The extract was phytochemical characterized using ultra-performance liquid chromatography, and total phenol and flavonoid analysis also was performed. The antioxidant capacity was determined through DPPH· radical, the antimicrobial property was evaluated against standard bacteria and fungi, and the viability assays were performed against mice splenocytes. Results: Fifteen compounds were identified belonging to two main classes terpenoids and phenolics. The extract showed 22.185 mg GAE/g of total phenolic compounds and 3.220 mg QE/g of flavonoid. Moreover, extract showed higher antioxidant ability similar to butylated hydroxytoluene a standard molecule [(3.042±0.019) mg AAE/g and (4.12±0.10) mg AAE/g to saline extract and butylated hydroxytoluene, respectively]. The antimicrobial assays demonstrated that the extract had a significant antifungal potential against Candida species and could be used with safety against mice splenocytes, in concentrations lower than 50 μg/mL, promoting higher proliferation in these cells. Conclusions: Saline extract from Tithonia diversifolia leaves presents potential antioxidant, antifungal properties and induces immunostimulation in mice splenocytes.
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