Figure 3: Immunohistochemical analyses by PAS staining. Immunohistochemistry was applied to compare the protective effects of O. humifusa aqueous extract via clinical observation or image analysis. (A) Dark violet blots indicate PAS-responsive cells. (B) Relative band intensities are shown in bar graph. Significant differences (P<0.05, ANOVA with Tukey post-hoc) when compared with the normal control (#) or the ethanol control (*) group. PAS: periodic acid Schiff.