Asian Pacific Journal of Tropical Biomedicine

ORIGINAL ARTICLE
Year
: 2020  |  Volume : 10  |  Issue : 4  |  Page : 147--155

A novel polyherbal formulation containing thymoquinone attenuates carbon tetrachloride-induced hepatorenal injury in a rat model


Aftab Ahmad1, Mohammed F Abuzinadah2, Huda M Alkreathy3, Hussam I Kutbi4, Noor Ahmad Shaik5, Varish Ahmad1, Shakir Saleem6, Asif Husain7 
1 Health Information Technology Department, Faculty of Applied Studies, King Abdulaziz University, Jeddah-21589, Kingdom of Saudi Arabia
2 Department of Medical Laboratory Technology, Faculty of Applied Medical Sciences, King Abdulaziz University, P. O. Box 80402 Jeddah-21589, Jeddah, Kingdom of Saudi Arabia
3 Department of Pharmacology, Faculty of Medicine, King Abdulaziz University, Jeddah, Saudi Arabia
4 Department of Pharmacy Practice, Faculty of Pharmacy, King Abdulaziz University, Jeddah, Saudi Arabia
5 Department of Genetic Medicine, Faculty of Medicine, King Abdulaziz University, Jeddah, Saudi Arabia
6 Department of Public Health, College of Health Sciences, Saudi Electronic University, Riyadh, Kingdom of Saudi Arabia
7 Department of Pharmaceutical Chemistry, School of Pharmaceutical Education and Research, Jamia Hamdard (Hamdard University), New Delhi, 110062, India

Correspondence Address:
Aftab Ahmad
Health Information Technology Department, Faculty of Applied Studies, King Abdulaziz University, Jeddah-21589
Kingdom of Saudi Arabia

Objective: To evaluate a novel polyherbal formulation (BSVT) containing the standardized extracts from the leaves of Boerhavia diffusa, Solidago virgaurea, Vitex negundo, and thymoquinone in CCl4 induced hepatorenal toxicity in rats. Methods: A total of 36 rats were divided into six groups including normal control, CCl4 (2 mL/kg, i.p.), CCl4 (2 mL/kg, i.p.) + Cystone® (750 mg/kgp.o.), CCl4 (2 mL/kg, i.p.) + BSVT (25 mg/ kg, p.o.), CCl4 (2 mL/kg, i.p.) + BSVT (50 mg/kg, p.o.), and CCl4 (2 mL/kg, i.p.) + BSVT (100 mg/kg,p.o.). All treatments were given for four weeks. Serum levels of aspartate transaminase, alanine transaminase, alkaline phosphatase, cholesterol, total protein, serum urea, blood urea nitrogen and creatinine were assessed. Superoxide dismutase, malondialdehyde, and glutathione peroxidase were evaluated in tissue homogenate. The histopathological study of liver and kidney tissues was also done. Results: Aspartate transaminase, alanine transaminase, alkaline phosphatase, cholesterol, serum urea, blood urea nitrogen and creatinine were significantly elevated (P<0.001) while total protein was considerably reduced in the CCl4 group as compared to the normal control (P<0.001), which indicated hepatorenal toxicity. In addition, superoxide dismutase and glutathione peroxidase activities were significantly decreased (P<0.001) while malondialdehyde levels were increased markedly (P<0.001). Treatment with BSVT formulation recovered these parameters towards a normal level in a dose-dependent manner. Conclusions: BSVT formulation ameliorates the hepatorenal toxicity in a dose-dependent manner. Furthermore, clinical studies are required to confirm its efficacy.


How to cite this article:
Ahmad A, Abuzinadah MF, Alkreathy HM, Kutbi HI, Shaik NA, Ahmad V, Saleem S, Husain A. A novel polyherbal formulation containing thymoquinone attenuates carbon tetrachloride-induced hepatorenal injury in a rat model.Asian Pac J Trop Biomed 2020;10:147-155


How to cite this URL:
Ahmad A, Abuzinadah MF, Alkreathy HM, Kutbi HI, Shaik NA, Ahmad V, Saleem S, Husain A. A novel polyherbal formulation containing thymoquinone attenuates carbon tetrachloride-induced hepatorenal injury in a rat model. Asian Pac J Trop Biomed [serial online] 2020 [cited 2020 May 25 ];10:147-155
Available from: http://www.apjtb.org/article.asp?issn=2221-1691;year=2020;volume=10;issue=4;spage=147;epage=155;aulast=Ahmad;type=0