Year : 2019 | Volume
: 9 | Issue : 8 | Page : 339--345
Proapoptotic activities of Oroxylum indicum leave extract in HeLa cells
Nurul Hidayah Wahab, Nur Afina Mohd Din, Yee Ying Lim, Noor Izani Noor Jamil, Nor Fazila Che Mat
School of Health Sciences, Health Campus, Universiti Sains Malaysia, 16150 Kubang Kerian, Kelantan, Malaysia
Objective: To examine the proapoptotic properties of Oroxylum indicum methanol extract on cervical cancer cells.
Methods: Methylene blue assay was used to determine the IC50 value of the extract. Western blotting assays were done to analyze the expression of HPV oncoproteins (HPV18 E6 and E7) and apoptotic molecules (caspase-3 and caspase-8). Reverse transcriptase PCR assays were performed to determine genetic alteration of tumor suppressors p53 and pRb and apoptosis markers Fas and FasL. Enzyme-linked immunosorbent assay (ELISA) was done to determine the expression of cytokine levels (IL-6 and IL-12).
Results: The determination of IC50 value indicated a higher anti-proliferative activity of the extract compared to cisplatin. After 24 hours of treatment, Western blot analysis showed that treated HeLa cells exhibited a significant down-regulation of HPV18 oncoproteins E6 and E7, and a significant induction of caspase-8 and caspase-3 activation level. Meanwhile, the mRNA expressions of p53, pRb, Fas and FasL were significantly upregulated in treated cells. Moreover, ELISA showed an increased IL-12 and decreased IL-6 production after Oroxylum indicum treatment.
Conclusions: The methanol extract of Oroxylum indicum has an anti-proliferative activity and proapoptotic potential. It induces localized-immunity improvements by altering cytokine production in HPV-positive cervical cancer cells.
Nor Fazila Che Mat
School of Health Sciences, Health Campus, Universiti Sains Malaysia, 16150 Kubang Kerian, Kelantan
|How to cite this article:|
Wahab NH, Mohd Din NA, Lim YY, Noor Jamil NI, Che Mat NF. Proapoptotic activities of Oroxylum indicum leave extract in HeLa cells.Asian Pac J Trop Biomed 2019;9:339-345
|How to cite this URL:|
Wahab NH, Mohd Din NA, Lim YY, Noor Jamil NI, Che Mat NF. Proapoptotic activities of Oroxylum indicum leave extract in HeLa cells. Asian Pac J Trop Biomed [serial online] 2019 [cited 2019 Oct 16 ];9:339-345
Available from: http://www.apjtb.org/article.asp?issn=2221-1691;year=2019;volume=9;issue=8;spage=339;epage=345;aulast=Wahab;type=0