Asian Pacific Journal of Tropical Biomedicine

BASIC RESEARCH
Year
: 2019  |  Volume : 9  |  Issue : 2  |  Page : 66--72

Fruiting increases total content of flavonoids and antiproliferative effects of Cereus jamacaru D.C. cladodes in sarcoma 180 cells in vitro


Jean Carlos Vencioneck Dutra1, Judá Ben-Hur de Oliveira1, Vanessa Silva dos Santos1, Paula Roberta Costalonga Pereira1, Jean Moisés Ferreira2, Maria do Carmo Pimentel Batitucci1 
1 Laboratório de Genética Vegetal e Toxicológica, Departamento de Ciências Biológicas, Universidade Federal do Espírito Santo, 29075-910, Vitória, Espírito Santo, Brazil
2 Laboratório de Biologia Molecular e Expressão Genica, Departamento de Ciências Biológicas, Universidade Federal de Alagoas, 57309-005, Arapiraca, Alagoas, Brazil

Correspondence Address:
Jean Carlos Vencioneck Dutra
Laboratòrio de Genética Vegetal e Toxicológica, Departamento de Ciências Biológicas, Universidade Federal do Espírito Santo, 29075-910, Vitória, Espírito Santo
Brazil

Objective: To evaluate the influence of fruiting phenological stage on total flavonoid content, antioxidant activity, and antiproliferative effects of Cereus jamacaru (C. jamacaru) (mandacaru) cladodes and fruit. Methods: Fruit and cladodes at vegetative and fruiting stage of C. jamacaru were collected. The fruit was dissected and bark, pulp, and seeds were separated. Vegetative and fruiting cladodes, together with bark, pulp, and seeds were used to obtain five hydroalcoholic extracts. The extracts were investigated for total flavonoid content, using AlCl3 colorimetric method, antioxidant activity by 2,2-diphenyl-1-picrylhydrazyl and 2,2’-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) radical scavenging capacity and Fe2+ ion chelating activity, and in vitro antiproliferative effects (sarcoma 180 cells) by 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide assay. Results: The extract of C. jamacaru cladodes at the fruiting stage showed higher flavonoid content compared to the other extracts. Seed extracts showed the highest antioxidant activity in 2,2-diphenyl-1-picrylhydrazyl and 2,2’-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) assays, and the extract of cladodes at vegetative stage showed better antioxidant activity in Fe2+ ion chelating activity. The extract of fruiting cladodes promoted higher antiproliferative effects compared to the other extracts. Conclusions: These findings suggest that fruiting increases the content of flavonoids and antiproliferative effects of C. jamacaru cladodes. Data reinforce the potential use of C. jamacaru cladodes and fruits as natural antioxidants and potent anticancer agent.


How to cite this article:
Dutra JC, de Oliveira JB, dos Santos VS, Pereira PR, Ferreira JM, Batitucci MC. Fruiting increases total content of flavonoids and antiproliferative effects of Cereus jamacaru D.C. cladodes in sarcoma 180 cells in vitro.Asian Pac J Trop Biomed 2019;9:66-72


How to cite this URL:
Dutra JC, de Oliveira JB, dos Santos VS, Pereira PR, Ferreira JM, Batitucci MC. Fruiting increases total content of flavonoids and antiproliferative effects of Cereus jamacaru D.C. cladodes in sarcoma 180 cells in vitro. Asian Pac J Trop Biomed [serial online] 2019 [cited 2019 May 23 ];9:66-72
Available from: http://www.apjtb.org/article.asp?issn=2221-1691;year=2019;volume=9;issue=2;spage=66;epage=72;aulast=Dutra;type=0