Asian Pacific Journal of Tropical Biomedicine

BASIC RESEARCH
Year
: 2018  |  Volume : 8  |  Issue : 1  |  Page : 19--24

Efficiency of combining pomegranate juice with low-doses of cisplatin and taxotere on A549 human lung adenocarcinoma cells


Nasser Mohamad1, Hijazi Akram1, Sayed Ahmad Bouchra2, Jamal Eddine Zeinab1, Ibrahim Sajida1, Rammal Hassan1, Al Rekaby Abd-El-Ameer3, Nasser Mohammed4 
1 Doctoral School of Science and Technology, Research Platform for Environmental Science (PRASE), Lebanese University, Lebanon
2 Doctoral School of Science and Technology, Research Platform for Environmental Science (PRASE), Lebanese University, Lebanon; Université Fédérale de Toulouse Midi-Pyrénées, INP-ENSIACET, Laboratoire de Chimie Agro-industrielle, Toulouse, France
3 Al Mustansiriya University, College of Science, Department of Biology, Iraq
4 American University of Beirut, Beirut, Lebanon

Correspondence Address:
Hijazi Akram
PhD researcher, Science of Agro-resources (EDST-EDSDM), Platforme de Recherche et d’Analyse en Sciences de l’Environnement, Hadath-Liban, Laboratoire de Chimie Agro-Industrielle, France

Sayed Ahmad Bouchra
PhD researcher, Science of Agro-resources (EDST-EDSDM), Platforme de Recherche et d’Analyse en Sciences de l’Environnement, Hadath-Liban, Laboratoire de Chimie Agro-Industrielle, France

Objective: To test the coalescence effect of two chemotherapy drugs at low effective dose (cisplatin and taxotere) combined with pomegranate juice on A549 cancer cells. Methods: Infrared spectroscopy method is a qualitative test that was performed to ensure the existence of the phytochemicals providing the antioxidant activity through the presence of the hydroxyl group (-OH). The viability of A549 cell line and normal MCs was tested using the neutral red uptake, Clonogenic survival, XTT and Cell migration assays. Results: Our results showed that this combination firstly led to a greater decrease in the viability of cells comparing to those treated with chemotherapy drugs alone, and secondly led to a significant reduction in cell migration. Conclusions: These data suggest a synergistic effect between the pomegranate and cisplatin which makes probably this combination a powerful option for treating lung adenocarcinoma and in parallel minimizing the systemic side effects.


How to cite this article:
Mohamad N, Akram H, Bouchra SA, Zeinab JE, Sajida I, Hassan R, Abd-El-Ameer A, Mohammed N. Efficiency of combining pomegranate juice with low-doses of cisplatin and taxotere on A549 human lung adenocarcinoma cells.Asian Pac J Trop Biomed 2018;8:19-24


How to cite this URL:
Mohamad N, Akram H, Bouchra SA, Zeinab JE, Sajida I, Hassan R, Abd-El-Ameer A, Mohammed N. Efficiency of combining pomegranate juice with low-doses of cisplatin and taxotere on A549 human lung adenocarcinoma cells. Asian Pac J Trop Biomed [serial online] 2018 [cited 2020 Feb 24 ];8:19-24
Available from: http://www.apjtb.org/article.asp?issn=2221-1691;year=2018;volume=8;issue=1;spage=19;epage=24;aulast=Mohamad;type=0