Asian Pacific Journal of Tropical Biomedicine

BASIC RESEARCH
Year
: 2018  |  Volume : 8  |  Issue : 12  |  Page : 586--592

Anti-cancer and anti-inflammatory activities of aronia (Aronia melanocarpa) leaves


Nhuan Do Thi, Eun-Sun Hwang 
 Department of Nutrition and Culinary Science, Hankyong National University, 327 Chungang-ro, Anseong-si, Gyeonggi-do, 456-749, Korea

Correspondence Address:
Eun-Sun Hwang
Department of Nutrition and Culinary Science, Hankyong National University, 327 Chungang-ro, Anseong-si, Gyeonggi-do, 456-749
Korea

Objective: To determine the anti-cancer effect of aronia leaf extract on SK-Hep1 cells using migration, metallo metrix proteinase-2/-9 (MMP-2/-9) and MT-1 MMP expression and to evaluate the anti-inflammatory activities of the leaf extract. Methods: The effect of aronia leaf extract on cancer prevention was investigated. SK-Hep1 human liver cancer cell line was treated with aronia leaf extract at various concentractions. MTT assay was used to measure cancer cell growth inhibition, and wound migration assay was used for metastasis determination. The expression of MMP-2/-9 was measured at the protein level using zymography and the expression of MMP-2/-9 and MT-1 MMP was examined at the gene level by RT-PCR. Raw 264.7 macrophage cells were stimulated with lipopolysaccharides to induce inflammation, and then the inhibition of inflammation was evaluated by treatment of aronia leaf extract. Expressions of interleukin-6, tumor factor-α, and nitric oxide (NO) were also determined. Results: SK-Hep1 cell growth was inhibited in proportion to the concentration of aronia leaf extract. In migration assay, aronia leaf extract showed 61.3%-96.3% wound size inhibtion after treating 50-200 μg/mL of aronia leaf extract for 24 h. At the protein level, the expression of MMP-2 and MMP-9 decreased as the concentration of aronia leaf extract treated with SK-Hep1 cells increased. In addition, the same pattern as in the protein was also observed in the mRNA levels. The expressions of MMP-2 and MMP-9 protein were inhibited by 92.2% and 53.8%, respectively after treatment with 200 μg/mL aronia leaf extract. In addition, Raw 264.7 cells treated with aronia leaf extract did not affect cell survival. There was dose dependent inhibition of interleukine-6, tumor necrosis factor-α and nitric oxide after treating aronia leaf extract in lipopolysaccharides-treated Raw 264.7 cell. Conclusions: The results show that aronia leaf has anticancer and and antimetastatic properties in SK-Hep1 and Raw 264.7 cells.


How to cite this article:
Thi ND, Hwang ES. Anti-cancer and anti-inflammatory activities of aronia (Aronia melanocarpa) leaves.Asian Pac J Trop Biomed 2018;8:586-592


How to cite this URL:
Thi ND, Hwang ES. Anti-cancer and anti-inflammatory activities of aronia (Aronia melanocarpa) leaves. Asian Pac J Trop Biomed [serial online] 2018 [cited 2019 Jan 23 ];8:586-592
Available from: http://www.apjtb.org/article.asp?issn=2221-1691;year=2018;volume=8;issue=12;spage=586;epage=592;aulast=Thi;type=0