Asian Pacific Journal of Tropical Biomedicine

BASIC RESEARCH
Year
: 2018  |  Volume : 8  |  Issue : 12  |  Page : 571--579

Gac fruit extracts ameliorate proliferation and modulate angiogenic markers of human retinal pigment epithelial cells under high glucose conditions


Ali Abdulqader1, Faisal Ali2, Amin Ismail3, Norhaizan Mohd Esa4 
1 Department of Nutrition and Dietetics, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia
2 Department of Nutrition and Dietetics, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia; Biochemistry & Molecular Biology Department, University Hospital, Faculty of Medicine and Health Sciences, Sana'a University, Yemen
3 Department of Nutrition and Dietetics, Faculty of Medicine and Health Sciences; Research Center of Excellent, Non-Communicable Diseases (NNCD) Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia
4 Department of Nutrition and Dietetics, Faculty of Medicine and Health Sciences; Research Center of Excellent, Non-Communicable Diseases (NNCD) Faculty of Medicine and Health Sciences; Laboratory of Molecular Biomedicine, Institute of Bioscience, Universiti Putra Malaysia, 43400, Serdang, Malaysia

Correspondence Address:
Prof. Amin Ismail
Research Center of Excellent, NNCD, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, 43400 Serdang, Selangor
Malaysia

Objective: To investigate the impact of the extracts of Gac fruit parts (peel, pulp, seed, and aril) on the cell viability and angiogenesis markers of human retinal pigment epithelial (ARPE- 19) cells under high glucose conditions. Methods: The effect of the extracts of Gac fruit peel, pulp, seed and aril on the ARPE-19 cells was determined using MTT viability assay, Trypan blue dye and morphological changes were observed using light microscopy. Enzyme-linked immunosorbent-based assay was performed to evaluate the effect of Gac fruit parts on the reactive oxygen species (ROS), vascular endothelial growth factor (VEGF) and pigmented epithelium-derived factor (PEDF) secretions. Results: High glucose (HG) at 30 mmol/L increased ARPE-19 cell viability and ROS and VEGF secretions. While, the exposure of ARPE- 19 cells in high glucose condition to Gac fruit extracts led to inhibition of cell viability, induced morphological changes, decreased ROS and VEGF secretions, and increased PEDF level. Gac pulp, seed, and aril at 1 000 μg/mL showed significant inhibition activities [(7.5 ± 5.1)%, (2.7 ± 0.5)%, (3.2 ± 1.1)%, respectively] against HG-induced ARPE-19 cell viability. The findings also demonstrated that Gac aril at 250 μg/mL significantly decreased ROS and VEGF levels [(40.6 ± 3.3) pg/mL, (107.4 ± 48.3) pg/mL, respectively] compared to ROS [(71.7 ± 2.9) pg/ mL] and VEGF [(606.9 ± 81.1) pg/mL] in HG untreated cells. Moreover, 250 μg/mL of Gac peel dramatically increased PEDF level [(18.2 ± 0.3) ng/mL] compared to that in HG untreated cells [(0.48 ± 0.39) ng/mL]. Conclusions: This study indicates that the extracts of Gac peel, pulp, seed and aril reduced cell viability, minimized ROS generations and showed angiogenic activities. Therefore, our findings open new insights into the potentiality of Gac fruit against HG-related diabetic retinopathy disease.


How to cite this article:
Abdulqader A, Ali F, Ismail A, Esa NM. Gac fruit extracts ameliorate proliferation and modulate angiogenic markers of human retinal pigment epithelial cells under high glucose conditions.Asian Pac J Trop Biomed 2018;8:571-579


How to cite this URL:
Abdulqader A, Ali F, Ismail A, Esa NM. Gac fruit extracts ameliorate proliferation and modulate angiogenic markers of human retinal pigment epithelial cells under high glucose conditions. Asian Pac J Trop Biomed [serial online] 2018 [cited 2019 Jun 20 ];8:571-579
Available from: http://www.apjtb.org/article.asp?issn=2221-1691;year=2018;volume=8;issue=12;spage=571;epage=579;aulast=Abdulqader;type=0