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ORIGINAL ARTICLE
Year : 2019  |  Volume : 9  |  Issue : 8  |  Page : 353-358

Cytotoxic effect of methanolic extracts of Fritillaria imperialis bulbs and Eryngium caucasicum leaves on hepatoma and colon cancer cells


1 Student Research Committee, Department of Immunology, Molecular and cell Biology Research Center, School of Medicine, Mazandaran University of Medical Sciences, Sari, Iran
2 Department of Immunology, School of Medicine, Mazandaran University of Medical Sciences, Sari, Iran
3 Pharmaceutical Sciences Research Center, Department of Medicinal Chemistry, Faculty of Pharmacy, Mazandaran University of Medical Sciences, Sari, Iran

Correspondence Address:
Alireza Rafiei
Department of Immunology, School of Medicine, Mazandaran University of Medical Sciences, Sari; 18 Km Khazar Blvd, Khazar Sq. Sari
Iran
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Source of Support: This work was supported by a grant of (grant no: 274-s-187) deputy of Research and Technology, Mazandaran University of Medical Sciences, Conflict of Interest: None


DOI: 10.4103/2221-1691.262084

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Objective: To evaluate antitumor activities of Fritillaria imperialis and Eryngium caucasicum methanolic extracts on human hepatoma (HepG2) and colon cancer (HCT116) cell lines in comparison to human foreskin fibroblasts as the normal cells. Methods: Methanolic extracts of Fritillaria imperialis and Eryngium caucasicum were prepared by the maceration method. The effect of the extracts at various concentrations (100, 200, 400, 600, and 800 μg/mL) on cell survival was evaluated using the MTT method. Besides, fluorescence staining was used to evaluate death patterns of the cells. Results: MTT assay showed that Fritillaria imperialis significantly decreased the viability of all cell lines after 24 and 48 hours of treatments. However, Eryngium caucasicum extract did not show any significant cytotoxicity effect on the cell lines. Fluorescence staining revealed that Fritillaria imperialis induced apoptosis of HCT116 cells at 550 μg/mL. Conclusions: Fritillaria imperialis extract has antiproliferative and cytotoxic effects on HCT116 and HepG2 cancer cells and therefore, may serve as an anticancer agent.


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