Cynodon dactylon extract ameliorates cognitive functions and cerebellar oxidative stress in whole body irradiated mice
Roopesh Poojary1, Nayanatara Arun Kumar M.Sc, PhD 1, Reshma Kumarchandra2, NA Vinodini1, K Bhagyalakshmi1, Ganesh Sanjeev3
1 Department of Physiology, Kasturba Medical College, Mangalore, Manipal Academy of Higher Education, Manipal, Karnataka, India
2 Department of Biochemistry, Kasturba Medical College, Mangalore, Manipal Academy of Higher Education, Manipal, Karnataka, India
3 Microtron Centre, Department of Studies in Physics, Mangalore University, Mangalagangotri, Karnataka, India
Dr. Nayanatara Arun Kumar
Associate Professor in Physiology, Department of Physiology, Kasturba Medical College, Mangalore, Manipal Academy of Higher Education, Manipal, Karnataka
Source of Support: This project was supported by the Board of Research in Nuclear Science
(BRNS) Mumbai, Department of Atomic energy, Government of India. [Grant
number No: 34 (1)/14/38/2014-BRNS/1932/27/11/2014], Conflict of Interest: None
Objective: To explore the effect of hydroalcoholic extract of Cynodon dactylon on the whole body radiation-induced oxidative status of the cerebellum and cognitive impairments in mice.
Methods: Swiss albino mice were randomly divided into the control group, radiation control group, low dose and high dose Cynodon dactylon extract treated groups and pre-treated with Cynodon dactylon extract before irradiation. Cynodon dactylon extract was administered for 7 d daily in low dose (0.25 g/kg) and high dose (1 g/kg). On day 7, mice were irradiated with a sublethal dose of 5 Gy gamma rays. Motor coordination was assessed by elevated rotarod test and spatial memory was studied by water maze test. Subsequently, biochemical markers (glutathione, lipid peroxidation and nitric oxide levels) in the cerebellum were evaluated.
Results: The gamma irradiated group showed significant impairment in motor coordination and spatial memory compared to normal mice. Mice treated by Cynodon dactylon extract prior to gamma radiation showed good improvement in both paradigms compared to the radiation control group. Moreover, glutathione level was increased, while lipid peroxidation and nitric oxide levels were significantly reduced in mice receiving low dose and high dose of Cynodon dactylon extract compared to the radiation control group.
Conclusions: The present study suggests the neuroprotective role of Cynodon dactylon against radiation-induced cognitive impairment and oxidative stress on the cerebellum of mice.