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ORIGINAL ARTICLE
Year : 2019  |  Volume : 9  |  Issue : 7  |  Page : 271-277

Pam3CSK4 enhances adaptive immune responses to recombinant Mycobacterium bovis bacille Calmette-Guérin expressing Plasmodium falciparum C-terminus merozoite surface protein-1


1 Biomedicine Programme, School of Health Sciences, Health Campus, Universiti Sains Malaysia, Kelantan, Malaysia
2 Biomedicine Programme, School of Health Sciences, Health Campus, Universiti Sains Malaysia, Kelantan, Malaysia; Department of Human Physiology, Faculty of Basic Medical Sciences, Bayero University Kano, Nigeria
3 Department of Medical Microbiology and Parasitology, School of Medical Sciences, Health Campus, Universiti Sains Malaysia, Kelantan, Malaysia

Correspondence Address:
Rapeah Suppian
Biomedicine Programme, School of Health Sciences, Health Campus, Universiti Sains Malaysia, Kelantan
Malaysia
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Source of Support: This work was supported by the Universiti Sains Malaysia (USM) Research University (RU) Grants (No. 1001/PPSK/8011100), Conflict of Interest: None


DOI: 10.4103/2221-1691.261742

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Objective: To determine the effects of toll-like receptor 2 (TLR-2) agonist, Pam3CSK4, on cellular and humoral immune response against recombinant Mycobacterium bovis bacille Calmette-Guérin (rBCG) expressing the C-terminus of merozoite surface protein-1 of Plasmodium falciparum. Methods: Six groups of mice (n=6 per group) received intraperitoneal phosphate buffered saline T80 (PBS-T80), BCG or rBCG in the presence or absence of Pam3CSK4. Enzyme- linked immunosorbent assay was carried out to measure serum total IgG, IgG1, IgG2a, and IgG2b production. Spleens were also harvested and splenocytes were co-cultured with rBCG antigen for in vitro determination of IL-4 and IFN- γ via enzyme-linked immunosorbent assay. Results: The production of total IgG and the isotype IgG1, IgG2a and IgG2b was significantly higher in rBCG-immunised mice than in the BCG and PBS-T80-immunised mice, and Pam3CSK4 further enhanced their productions. A similar pattern was also observed in IFN- γ production. Moreover, there was no significant difference in IL-4 production in all groups either in the presence or absence of Pam3CSK4. Conclusions: We present evidence of the adjuvant effects of TLR-2 agonist in enhancing the production of total IgG, IgG1, IgG2a, IgG2b, as well as IFN- γ in response to rBCG. However, the presence or absence of Pam3CSK4 had no effect on IL-4 production.


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