A new angiotensin-converting enzyme inhibitor from Peperomia pellucida (L.) Kunth
Islamudin Ahmad1, Neneng Siti Silfi Ambarwati2, Berna Elya3, Hanita Omar4, Kamarza Mulia5, Arry Yanuar3, Osamu Negishi6, Abdul Mun´im3
1 Department of Pharmaceutical Sciences, Faculty of Pharmacy, Mulawarman University, Samarinda, East Kalimantan, Indonesia
2 Department of Cosmetology, Engineering Faculty, Universitas Negeri Jakarta, East Jakarta, Indonesia
3 Department of Pharmaceutical Sciences, Faculty of Pharmacy, Universitas Indonesia, Depok, West Java, Indonesia
4 Chemistry Division, Centre for Foundation Studies in Science, University of Malaya, Kuala Lumpur, Malaysia
5 Department of Chemical Engineering, Faculty of Engineering, Universitas Indonesia, Depok, West Java, Indonesia
6 Department of Applied Biochemistry, Faculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba, Ibaraki, Japan
Gedung Administrasi Fakultas Farmasi Kampus Unmul Gunung Kelua, Jalan Panajam, Samarinda, East Kalimantan
Source of Support: It is supported by grant “Hibah Tugas Akhir Mahasiswa Doktor (TADOK)
Tahun 2018” Directorate of Research and Humanity Engagement Universitas
Indonesia (grant number: 1234/UN2.R3.1/HKP.05.00/2018), Conflict of Interest: None
Objective: To isolate, identify, and evaluate a new angiotensin-converting enzyme inhibitor from Peperomia pellucida (L.) Kunth herbs.
Methods: A dried sample of Peperomia pellucida herb was successively macerated with n-hexane and ethyl acetate. The ethyl acetate extract solution was evaporated to obtain the crude extract. Vacuum liquid column chromatography and thin layer chromatography were performed to obtain two pure compounds. Then, both compounds were elucidated and identified using the spectroscopic method. Angiotensin-converting enzyme inhibitory activity studies of both compounds were determined using angiotensin-converting enzyme kit WST-1 with spectrophotometer microplate reader 96-well at 450 nm wavelength.
Results: Two bioactive compounds were successfully isolated from Peperomia pellucida herb, including a new compound of 2,3,5-trimethoxy-9-(12,14,15-trimethoxybenzyl)-1H-indene and pellucidin A. Both compounds demonstrated angiotensin-converting enzyme inhibitory activity, with IC50 values of 72 μM (27.95 μg/mL) and 11 μM (4.4 μg/mL), respectively.
Conclusions: In the present study, two active angiotensin-converting enzyme inhibitors were successfully isolated and purified from Peperomia pellucida which is used as an antihypertensive in traditional medicine, and support its use as an angiotensin-converting enzyme-inhibiting drug.