Impact Factor 2018 : 1.587 (@Clarivate Analytics)
  • Users Online: 636
  • Print this page
  • Email this page
BASIC RESEARCH
Year : 2019  |  Volume : 9  |  Issue : 3  |  Page : 129-134

Sequence analysis of Turkish field strains of bovine torovirus shows unique amino acid changes in the partial M gene


Department of Virology, Faculty of Veterinary, University of Ataturk, Erzurum-25240, Turkey

Correspondence Address:
Hakan Aydin
Atatürk University, Faculty of Veterinary Medicine, Department of Virology, TR-25240 Erzurum
Turkey
Login to access the Email id

Source of Support: None, Conflict of Interest: None


DOI: 10.4103/2221-1691.254607

Get Permissions

Objective: To investigate the presence, prevalence and phylogenetic classification of bovine torovirus (BToV). Methods: Stool samples from 72 calves, which were negative for primary gastroenteritis agents, were examined with the nested PCR method by using BToV M gene-specific primers. Results: BToV was detected in 12 (16.7%) out of 72 samples. Phylogenetic analysis was performed using nucleotide and amino acid sequences. In the phylogenetic tree, European, American, Far East and Turkish strains were found to be divided into different branches. Interestingly, it was observed that Turkish strains were divided into two subgroups. Considering the amino acid sequences of these strains having differences at nucleotide level, the change at the 3rd amino acid of the partial M gene in Turkish strains has made Turkish strains different from all other strains. Similarly, the differences were observed in the 18th, 20th, 63rd and 93rd amino acids of the partial M gene only in Turkish field strains. Conclusions: This study revealed that Turkish strains of BToV constitute a separate phylogenetic group and can be divided into two subgroups. In addition, BToV was found to be a common pathogen causing diarrhea in calves in Turkey, and it is a necessity to consider BToV in cases of diarrhea with unknown cause.


[FULL TEXT] [PDF]*
Print this article     Email this article
 Next article
 Previous article
 Table of Contents

 Similar in PUBMED
   Search Pubmed for
   Search in Google Scholar for
 Related articles
 Citation Manager
 Access Statistics
 Reader Comments
 Email Alert *
 Add to My List *
 * Requires registration (Free)
 

 Article Access Statistics
    Viewed236    
    Printed27    
    Emailed0    
    PDF Downloaded84    
    Comments [Add]    

Recommend this journal