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BASIC RESEARCH
Year : 2019  |  Volume : 9  |  Issue : 2  |  Page : 60-65

In vitro evaluation of anti-acetylcholinesterase and free radical scavenging potential of leaf extracts of some selected medicinal plants


1 Department of Bio-Engineering, Birla Institute of Technology, Mesra, Ranchi, Jharkhand, India
2 Department of Pharmaceutical Sciences and Technology, Birla Institute of Technology, Mesra, Ranchi, Jharkhand, India

Correspondence Address:
Sheela Chandra
Department of Bio-Engineering, Birla Institute of Technology, Mesra, Ranchi, Jharkhand-835215
India
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/2221-1691.250856

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Objective: To evaluate the phytochemical present in various solvent extracts from leaves of Ocimum sanctum (L.), Swertia chirayita (L.), Butea monosperma (Lam.) and Stevia rebaudiana (Bert.) as well as antioxidant and anticholinergic activities employing different in vitro models. Methods: Total phenol content of diethyl ether, chloroform and methanolic extracts obtained from leaves of different medicinal plants was determined by Folin-Ciocalteau's spectrophotometric method. Moreover, antioxidant and anticholinergic studies were conducted by four different in vitro methods which included diphenyl picrylhydrazyl radical scavenging, 2,2-azinobis (3-ethylbezoline-6-sulphonic acid), reducing activity by ferrous reduced antioxidant power and anti-acetylcholinesterase assay, in order to ensure pharmacological potential of the plants. Results: The methanolic leaf extract of Ocimum sanctum showed the highest total phenol content which was (21.13±1.04) GAE/g DW and antioxidant activities compared to other plants with the IC50 value of 40.43 μg/mL in diphenyl picrylhydrazyl radical scavenging assay and 53.5 μg/mL in 2,2-azinobis (3-ethylbezoline-6-sulphonic acid) assay as well as metal ion reduced by (78.22±0.38) TE/g DW in ferrous reduced antioxidant power assay. The inhibition percentage of the anti-acetylcholinesterase assay was (94.22±0.26)%. Conclusions: The results of our current study show that Ocimum sanctum leaf is the most significant source of phytochemicals that possesses antioxidant and anticholinergic properties. However, further investigation on isolation and characterization of active compound which is responsible for the pharmacological potential is needed.


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