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ORIGINAL ARTICLE
Year : 2019  |  Volume : 9  |  Issue : 11  |  Page : 456-466

Antidiabetic activity of Callicarpa nudiflora extract in type 2 diabetic rats via activation of the AMPK-ACC pathway


1 Key Laboratory of Xinjiang Phytomedicine Resource and Utilization for Ministry of Education, School of Pharmacy, Shihezi University, Shihezi 832000; Key Laboratory of South Subtropical Plant Diversity, Fairy Lake Botanical Garden, Shenzhen & Chinese Academy of Sciences, Shenzhen 518004, China
2 Key Laboratory of Xinjiang Phytomedicine Resource and Utilization for Ministry of Education, School of Pharmacy, Shihezi University, Shihezi 832000, China
3 Department of Pharmacy, 928 Hospital of PLA, Haikou 571159, China
4 Key Laboratory of South Subtropical Plant Diversity, Fairy Lake Botanical Garden, Shenzhen & Chinese Academy of Sciences, Shenzhen 518004, China

Correspondence Address:
Li-Ping Tian
Key Laboratory of Xinjiang Phytomedicine Resource and Utilization for Ministry of Education, School of Pharmacy, Shihezi University, Shihezi 832000
China
Shi-Xiu Feng
Key Laboratory of South Subtropical Plant Diversity, Fairy Lake Botanical Garden, Shenzhen & Chinese Academy of Sciences, Shenzhen 518004
China
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Source of Support: This work is supported by the Key Research & Development Plan of Hainan Province (No. ZDYF2018228), and Natural Science Foundation of Guangdong Province (No.2016A030313034), Conflict of Interest: None


DOI: 10.4103/2221-1691.270978

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Objective: To evaluate the antidiabetic effect of Callicarpa nudiflora extract in streptozotocin- induced diabetic rats. Methods: The chemical constituents in Callicarpa nudiflora extract were identified by UPLC- Q-TOF-MS. Callicarpa nudiflora extract (0.15 and 0.3 g/kg) was orally administered to streptozotocin-induced diabetic rats for 42 d. The effects of Callicarpa nudiflora extract on body weight, blood glucose, serum insulin, total cholesterol, triglyceride, LDL-C and HDL-C were investigated, and its effect on liver and pancreatic pathology was assessed by histopathological analysis. Moreover, the expression levels of adenosine 5’-monophosphate-activated protein kinase (AMPK), glucose transporter type 4 (GLUT4), phospho-AMPK/AMPK, and p-acetyl- coA carboxylase (P-ACC)/ACC in the skeletal muscles and liver were determined by reverse transcription-polymerase chain reaction, Western blotting, and immunohistochemistry. Results: A total of 34 compounds, including 8 iridoids, 14 phenylpropanoids, and 12 flavonoids, were identified from Callicarpa nudiflora extract. Callicarpa nudiflora extract significantly reduced blood glucose and significantly restored all other biochemical parameters to near normal levels, including serum insulin, total cholesterol, triglyceride, LDL-C, and HDL-C. Callicarpa nudiflora extract improved insulin resistance and reversed the damage in the liver and pancreas caused by diabetes. Furthermore, Callicarpa nudiflora extract increased the expression levels of phospho-AMPK, GLUT4, P-ACC, and insulin receptor substrate-1 and decreased the expression level of PPAR γ in diabetic rats. Conclusions: Callicarpa nudiflora extract improved oral glucose tolerance, lipid metabolism, insulin resistance, and reversed the diabetes-related damage in the liver and pancreas by activating the AMPK-ACC pathway.


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