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ORIGINAL ARTICLE
Year : 2019  |  Volume : 9  |  Issue : 11  |  Page : 449-455

Antidiarrheal potential of Distemonanthus benthamianus Baillon. extracts via inhibiting voltage-dependent calcium channels and cholinergic receptors


1 Research Unit of Animal Physiology and Phytopharmacology, Faculty of Science, University of Dschang, PO. Box 67 Dschang, Cameroon; Department of Pharmacy, COMSATS University Islamabad, Abbottabad Campus 22060, Pakistan
2 Research Unit of Animal Physiology and Phytopharmacology, Faculty of Science, University of Dschang, PO. Box 67 Dschang, Cameroon
3 Department of Pharmacy, COMSATS University, Islamabad, Abbottabad Campus 22060, Pakistan
4 Laboratory of Animal Physiology, Faculty of Science, University of Yaoundé 1, PO. Box 812 Yaoundé, Cameroon

Correspondence Address:
Gilbert Ateufack
Research Unit of Animal Physiology and Phytopharmacology, Faculty of Science, University of Dschang, PO. Box 67 Dschang
Cameroon
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Source of Support: This work was supported by the CIIT-TWAS Sandwich Postgraduate Fellowship (FR number: 3240293217, 2016), Conflict of Interest: None


DOI: 10.4103/2221-1691.270977

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Objective: To evaluate spasmolytic mechanisms of aqueous and methanolic extracts from Distemonanthus benthamianus trunk-bark. Methods: Spasmolytic activities of extracts were evaluated in vitro on spontaneous and potassium chloride-induced jejunum contractions, or against cholinergic [acetylcholine (0.3 μmol/L)] stimulations. High performance liquid chromatography analysis of both extracts was performed in reference to standard compounds. Results: Extracts developed concentration-dependent inhibitory activities. The methanolic extract, which revealed better activity, produced spasmolytic and myorelaxant effects at concentrations of 0.01-0.30 mg/mL with EC50 of 0.06 and 0.09 mg/mL (95% CI: 0.03-0.3 mg/mL), respectively. Its anticholinergic effect was obtained at the same concentrations with EC50 of 0.11 mg/mL (95% CI: 0.03-0.3 mg/mL). Chromatograms showed the presence of gallic acid in both extracts, rutin being only detected in the aqueous extract. Conclusions: Distemonanthus benthamianus extracts exhibit verapamil and atropine-like activities, thus highlighting calcium channels and muscarinic receptors blocking potentials, which may be conveyed by some phenolic compounds. These results confirm the antidiarrheal activity of Distemonanthus benthamianus extracts.


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