|Year : 2018 | Volume
| Issue : 4 | Page : 207-216
Phenological stage effect on phenolic composition and repellent potential of Mentha pulegium against Tribolium castaneum and Lasioderma serricorne
Nidhal Salem1, Jazia Sriti1, Olfa Bachrouch2, Kamel Msaada1, Saber Khammassi1, Majdi Hammami1, Saoussen Selmi1, Emna Boushih2, Marwa Ouertani1, Nesrine Hachani1, Manef Abderraba3, Brahim Marzouk1, Ferid Limam1, Jouda Mediouni Ben Jemaa4
1 Laboratoire des Substances Bioactives, Centre de Biotechnologie à la Technopole de Borj Cedria, BP, 901, 2050 Hammam Lif, Tunisia
2 Laboratoire de Protection des Végétaux, Institut National de la Recherche Agronomique de Tunisie (INRAT), Rue Hedi Karray, 2049 Ariana, Tunis, Université de Carthage, Tunisia
3 Institut Supérieur des Sciences Biologiques Appliquées de Tunisia, Tunis, Tunisia
4 Laboratoire de Biotechnologie Appliquée à l'Agriculture, Institut National de la Recherche Agronomique de Tunisie (INRAT), Rue Hedi Karray, 2049 Ariana, Tunis, Université de Carthage, Tunisia
|Date of Submission||17-Nov-2017|
|Date of Decision||05-Mar-2018|
|Date of Acceptance||10-Apr-2018|
|Date of Web Publication||30-Apr-2018|
Laboratoire des Substances Bioactives, Centre de Biotechnologie à la Technopole de Borj Cedria, BP, 901, 2050 Hammam Lif
Source of Support: None, Conflict of Interest: None
Objective: To elucidate for the first time the phenological stage effect on polyphenol, flavonoid and proanthocyanidin contents of pennyroyal (Mentha pulegium) as well as their antioxidant capacities and insecticidal potentials against Tribolium castaneum and Lasioderma serricorne. Methods: Crude methanol extracts from pennyroyal (Mentha pulegium) aerial parts were evaluated for their antiradical, reducing power activities and repellent potentials against selected coleopteran insects during different phenological stages. Results: Phenolic contents of pennyroyal aerial parts and their antioxidant effects were significantly dependent on maturity stage of the plant. The maximum of phenol production was reached during the full flowering stage. Apigenin was the major phenol in Mentha pulegium with an amount of 6.01 mg/g of dry vegetable matter during this period. Nevertheless, at fructification stage, the highest antioxidant activity was not related to high phenolic content. The repulsive effect of pennyroyal extract showed that Lasioderma serricorne was more susceptible to pennyroyal extract than Tribolium castaneum with corresponding median repellent dose values of 0.124 and 0.006 mg/mL respectively. Moreover, great differences in insect repulsion depending on extract concentrations, exposure time and developmental stage was observed. For the lowest concentration (0.125 mg/mL), the repellent effect against Tribolium reached its maximum (90%) during the fructification stage (after 24 h of exposure) while this repellent effect was 80% during the vegetative stage. Conclusions: Due to the strong insecticidal potential of pennyroyal extracts, this study highlights the therapeutic properties of this plant and encourages its use as a safer, environmental-friendly and efficient insecticide in food industry.
Keywords: Apigenin Lasioderma, Repellent activity, Full flowering stage
|How to cite this article:|
Salem N, Sriti J, Bachrouch O, Msaada K, Khammassi S, Hammami M, Selmi S, Boushih E, Ouertani M, Hachani N, Abderraba M, Marzouk B, Limam F, Ben Jemaa JM. Phenological stage effect on phenolic composition and repellent potential of Mentha pulegium against Tribolium castaneum and Lasioderma serricorne. Asian Pac J Trop Biomed 2018;8:207-16
|How to cite this URL:|
Salem N, Sriti J, Bachrouch O, Msaada K, Khammassi S, Hammami M, Selmi S, Boushih E, Ouertani M, Hachani N, Abderraba M, Marzouk B, Limam F, Ben Jemaa JM. Phenological stage effect on phenolic composition and repellent potential of Mentha pulegium against Tribolium castaneum and Lasioderma serricorne. Asian Pac J Trop Biomed [serial online] 2018 [cited 2018 May 23];8:207-16. Available from: http://www.apjtb.org/text.asp?2018/8/4/207/231283
Foundation project: This work was funded by the Ministry of Higher Education and Scientific Research in Tunisia.
| 1. Introduction|| |
Despite the importance of the majority of insects for humanity as producers of pharmacologically active compounds such as venoms or antibodies, coleopterans constitute a real threat and a serious risk to the world. In fact, these insects are often found infesting food ingredients where they cause damage by piercing the packages, leading to grain deterioration and mycotoxin generation. The damaged products then become unfit for human consumption and must be removed from the food production chain,.
The small reddish brown coleopteran [Tribolium castaneum Herbst (T. castaneum)] (Tenebrionidae) constitutes the major beetle of preserved grain throughout the world. The easy adaptability to survive harsh environments allows it to colonize different biotic and abiotic surfaces. The tobacco pest, Lasioderma serricorne (L. serricorne) is a species of beetle insects of Anobiidae family. It is very similar to the worm of bread (Stegobium paniceum) and the little vrillette (Anobium punctatum). This insect causes damage in many herbs and spices.
Damage inflicted by these insects could be overcome by pesticides. However, synthetic insecticides may pose possible health hazards to human and environment. Indeed, the repeated treatments of these chemical products may cause insecticide resistance in beetles. In another hand, humanity is facing to severe threat from pesticides overusing. In fact, their drawbacks were coupled with neurotoxicity damages and neurodegenerative disorders by interfering with chemical neurotransmission, leading to lethal consequences,.
As a result, an increasing attention was turned in developing better alternatives to synthetic insecticides. With respect to biological control, plant-based extracts have been suggested to provide potential insect-control agent. In this context, plant extracts contain complex mixture of secondary metabolites and natural dyes such as phenols which act as repellents, feeding deterrents and toxins.
Phenols such as quinine, lignin and salicylates are among the best-known compounds tested against insects. The defensive mechanism of these compounds consists of limiting pathogens entrance by increasing the leaf toughness. Moreover, phenol oxidation activated by peroxidase would be considered as the ultimate defense against these pests. Of the same, flavonoids (such as flavonols, flavones, flavan 3-ols, flavonones, and isoflavonoids) and proanthocyanidins constitute other offensive agents against insects. Thanks to their cytotoxicity, enzymatic complexation and astringency, these phenolic compounds act as feeding deterrents and preventing attack from many phytophagous insects.
In Tunisia, several herbs can act as sources of these natural repellents, including pennyroyal [Mentha pulegium L. (M. pulegium); Lamiaceae]. This plant, known as Fliou, is one of the species frequently used in aromatherapy science among four other mint varieties: Mentha rotundifolia, Mentha longifolia, Mentha spicata and Mentha aquatica. Aerial parts of M. pulegium were characterized by a wide diversity of bioactive components such as tannins, resins, pectins, polyphenols and essential oils. These constituents exhibited interesting biological activities such as antispasmodic, antihaemorrhoidal, sedative, hypotensive, anti-inflammatory, analgesic, antimicrobial and insecticidal activities.
Since there are no published reports concerning the effect of phenological stage on the insecticidal potentials of M. pulegium extracts until now, this study was undertaken. Hence, the common target of this research was to ascertain the potential impact of phenological stages on polyphenol, flavonoid and proanthocyandin contents and their antioxidant activity (i), to determine the profiles of different phenolic compounds and quantify them (ii) and to assess the repellent potentials of M. pulegium extracts against T. castaneum and L. serricorne during its vegetable stages (iii). Such a work is encouraging in order to recommend the use of M. pulegium as a safe, convenient, and low-cost alternative bioinsecticide instead of harmful pesticides.
| 2. Materials and methods|| |
2.1. Chemical reagents
The reagents used were sodium hydroxide (NaOH), chlorhydric acid (HCl), disodium hydrogen phosphate (Na2HPO4), sodium monobasic phosphate (NaH2PO4H2O), sodium carbonate (Na2CO3), sodium nitrite (NaNO2), butylated hydroxytoluene (BHT), 1,1-diphenyl-2-picrylhydrazyl (DPPH), potassium ferricyanide [K3Fe(CN)6], trichloroacetic acid (TCA), ferric chloride solution, ascorbic acid, polyvinyl polypyrolidone Folin Ciocalteu reagent, potassium ferricyanide [K3Fe(CN)6] and aluminum chloride (AlCl3). All these products were provided from Sigma Aldrich (Steinheim, Germany). Phenolic authentic standards were purchased from Fluka. Stock solutions of these compounds were prepared in high performance liquid chromatography (HPLC)-grade methanol, conserved in aluminum glass vials and stored at -20 °C. Methanol used was of analytical grade and delivered from laboratory reagents and fine chemicals.
2.2. Vegetal collection
To ensure homogeneous sampling, M. pulegium (leaves, stems and flowers) were collected randomly from Oued el Abid region [latitude 36° 52' 52.19''(N); longitude 10° 42' 26.41''(E), altitude 1 060 m)] at three phenological stages; vegetative (ST1), full flowering (ST2) and fructification (ST3) stages. The collection was done three times. The plants were identified by the botanist Abderrazak (Professor in Biotechnology Center in Borj Cedria Technopole, Tunisia) according to the Tunisian flora. We prepared the voucher specimen in laboratory of bioactive substances for further studies. In presence of nitrogen, pennyroyal samples were freeze-dried and ground to fine powder. After that, they were conserved in a dessicator at room temperature (~25 °C) in darkness for later use.
2.3. Phenol extraction
The dried samples were milled in an electrical stainless steel blender and placed at room temperature away from moisture. Concerning the extraction by Soxhlet, a quantity of 20 g of vegetable powder (aerial part) were placed in a cartridge in the presence of 200 mL of methanol. The extraction lasted 8 h. The extract was recovered, filtered (Wattman # 4), concentrated by rotary evaporator and stored at 4 °C.
2.4. Total polyphenol content
According to the extraction protocol described by Dewanto et al. Quantitative estimation of polyphenols was carried out using the Folin ciocalteu reagent. Different concentrations of stock solution were prepared. For each one was added to 125 μ L of Folin Ciocalteu reagent. After 2 min, 1 250 μ L of Na2CO3 (7 g/100 mL) were supplied to promote an alkaline medium and to start the oxidation-reduction reaction. After adjusting with distilled water to a final volume of 3 mL, these extract solutions were kept in the dark for 90 min at room temperature. The absorbance of each extract solution was read as described at 760 nm.
Gallic acid was used as a standard to estimate the total amount of polyphenols.
2.5. Total flavonoid content
The flavonoid assay was performed by a method based on the formation of complex between phenolic compounds and AlCl3. From the stock solution of pennyroyal extracts prepared in methanol, different dilutions ranging from 25 to 500 μ g/mL were prepared. A total of 0.125 mL of each solution was added to 75 μ L of NaNO2 solution (5%) and 0.15 mL of AlCl3 (10%). All were mixed thoroughly for 6 min. Obtained mixture was then added to 0.5 mL of 1 M NaOH solution. The assay was carried out by UV/Visible spectrophotometry at 510 nm and total flavonoid content of pennyroyal aerial parts was expressed as mg quercetin equivalents (QE) per gram of dry weight (mg QE/g DW) through its calibration curve.
2.6. Proanthocyanidin content
The colorimetric method was based on the oxidative cleavage of proanthocyanidins with hydrochloric acid showing a red color proportional to the quantity of these compounds. A volume of 0.5 mL of methanolic extract was added to a volume of 3 mL of vanillin solution (4%) and 1.5 mL of HCl. After 15 min, the absorbance was read at 500 nm and the concentration of proanthocyanidins was expressed in quercetin equivalent (mg QE/g DW).
2.7. Phenolic profile analysis of pennyroyal extracts by RP–HPLC
Twenty μ L of pennyroyal extracts were analyzed on a HPLC system (Agilent 1260, Agilent technologies, Germany). This method consisted on a vacuum degasser, an autosampler and a binary pump with a maximum pressure of 400 bar. The phenolic compounds were separated on a reversed phase C18 analytical column (Zorbax Eclipse XDB, 4.6 mm x 100 mm, 3.5 μ m particle size) at a temperature set at 25 °C. The chromatograms obtained at 280, 320 and 550 nm (DAD detector, Germany) were compared with those obtained on known phenolic compound standards. The separation gradient was formed by two mobile phases: A, methanol and B, 99.9% H2O and 0.1% formic acid. The optimized gradient elution was illustrated as follows: 0-5 min, 10%-20% A; 5-10 min, 20%-30% A; 10-15 min, 30%-50% A; 15-20 min, 50%-70% A; 20-25 min, 70%-90% A; 25-30 min, 90%-50% A; 30-35 min. Phenolic compound contents were expressed in mg/g DW.
2.8. Antioxidant capacity evaluation
2.8.1. Antiradical scavenging potential
In this analysis, the purple-colored DPPH was reduced by the so-called antioxidants into pale yellow hydrazine. So antioxidant effect on this radical translated its ability to give hydrogen radical. Two thousand μ L of each methanolic extract at different concentrations (0.1-100 μ g/mL) were added to 500 μ L of a methanolic solution from DPPH at 0.2 mM. After incubation in the dark for 30 min and at room temperature, the reading of absorbances was carried out at 517 nm using a spectrophotometer.
We thus calculated the percentages of inhibition by the following formula:
IP% = [(Ac-At)/Ac]×100
Where IP% was inhibition percentage, Ac absorbance of control and At absorbance of the test made. BHT was used as positive control at the same concentrations as the tested extracts.
The semi-logarithmic curve obtained made it possible to establish the IC50 of the sample which corresponded to the concentration leading to 50% of DPPH radical inhibition.
2.8.2. Ferric reducing antioxidant power
The reducing power of an extract was associated with its antioxidant power. The reducing ability of our extracts was determined according to the method of Oyaizu (1986). This method was based on the chemical reaction to reduce ferric iron (Fe3+) present in the complex K3Fe(CN)6 into ferrous iron (Fe2+). To 1 mL of the sample at different concentrations (50, 200, 1 000 and 2 000 μ g/mL), 2.5 mL of a phosphate buffer solution (0.2 M, pH 6.6) and 2.5 mL of K3Fe(CN)6 at 1% were added. The mixture was incubated at 50 °C for 20 min and then cooled at room temperature. About 2.5 mL of TCA (10%) was added to stop the reaction, then the tubes were centrifuged at 3 000 rpm for 10 min. Then 2.5 mL of the supernatant were added to 2.5 mL of distilled water and 500 μ L of a 0.1% solution of FeCl3•6H2O. The absorbances were read against a blank at 700 nm using a spectrophotometer. An increase in the absorbance corresponded to an increase in the reducing power. So, the concentration providing 0.5 of absorbance (EC50, μ g/mL) was calculated directly from the graph of absorbance. Ascorbic acid was used as a positive control in this experiment under the same conditions.
2.9. Insecticidal activity
2.9.1. Pest rearing
The experiment was carried out in the laboratory of plant protection at the National Institute of Agricultural Research in Tunisia on two strains; the red floor beetle T. castaneum and the cigarette beetle L. serricorne. This choice of animal material was justified by the importance of the damage of these pests, which infested stored foodstuffs of economic importance. These insects were easily reared in the laboratory in glass jars on wheat floor (Triticum aestivum) without exposure to insecticides. All these jars were placed in a dark oven set at a temperature of (25±1) °C, a relative humidity of (65±5)% and 15 h:9 h (light: dark) photoperiod, which were the optimal conditions for the development of these insects. It should be noted that pests used for repellent testing were 7-10 days old.
2.9.2. Repellency bioassay
A single insecticidal test was assessed for the extracts during different stages of M. pulegium against two beetle species: T. castaneum and L. serricorne
This test was used to study the repulsive effect of the extract tested against the two beetles by determining the percentage of repulsion of each extract as well as the median dose of repulsion. The repellent effect of M. pulegium extracts on coleopteran was evaluated using the preferential filter paper method described by Jilani and Saxena. Thus, the 8 cm diameter Wattman filter paper discs were used. For this purpose, they were cut into two equal parts. Four concentrations of extracts were prepared from the stock solution (Ss) [C1 (Ss), C2 (1/2 Ss), C3 (1/4 Ss), and C4 (1/8 Ss)] by dilution in acetone. Then 0.150 mL of each dilution thus prepared was spread uniformly over one half of the disc while the other half received only 0.150 mL of acetone. Five repetitions were performed for each concentration. After fifteen min which was time required for complete evaporation of solvent, the two halves of the disks were attached using an adhesive tape. The reconstituted filter paper disc was placed in a petri dish and 20 insects of mixed sex (ratio 1:1) were placed in the center of each disc.
The mortality monitoring of the insects on the petri dish was carried out according to a kinetics of 1 h up to 24 h for each tested concentration.
Number of insects (L. serricorne and T. castaneum) presented on the part of filter paper treated with pennyroyal extract (Nt) and the number of those presented on the part treated only with acetone (Nc) were recorded. The percentage of repulsion (PR) was calculated using the formula of Nerio et al as bellow:
PR = [(Nc-Nt)/(Nc+Nt)] × 100
The mean repellency value of each extract was calculated and assigned to repellency classes from 0 to V.
Class 0: The percentage of repulsion ≤ 0.1%: not repulsive
Class I: 0.1%<PR<20%: very poorly repulsive
Class II : 20.1%<PR≤40%: weakly repulsive
Class III: 40.1%<PR≤60%: moderately repulsive
Class IV: 60.1%<PR≤80%: repulsive
Class V: 80.1%<PR≤100%: very repulsive.
Probit analysis was used to calculate the median repellent dose (RD50) (dose that repelled 50% of the exposed insects) and RD95 (dose that repelled 95% of the exposed insects) at 24 h of exposure. Results were presented as the mean of percentage repellency ± the standard error.
2.10. Statistical analysis
The results obtained were analyzed statistically using STATISTICA software and expressed as mean ± SD. Then, they were discriminated against Duncan's test at threshold of 5%. Probit analysis was conducted to estimate repellent doses (RD50 and RD95) with their 95% fiducial limits. Correlation coefficients were calculated between total polyphenol, total flavonoids, proanthocyanidins and their antioxidant activities (DPPH and reducing power) based on their contents.
| 3. Results|| |
3.1. Extraction yield
Extraction yield varied significantly (P<0.05) as a function of the development stage of M. pulegium. In fact, higher extraction yield was reached at ST2 stage (19.34%), followed by that at ST1 stage (14.23%). Whereas, ST3 stage showed the lowest extraction yield with a value of 11.11%.
3.2. Phenological effect on phenolic contents
Phenolic quantification (polyphenols, flavonoids and proanthocyanidins) depending on harvest time was given by [Table 1]. Indeed, polyphenol level reached its maximum during the full flowering stage (ST2) with a content of 37.67 mg GAE/g DW. Beyond this stage, the level decreased to a value of 22.19 mg GAE/g DW.
|Table 1: Total polyphenol, flavonoid and proanthoyanidin contents of M. pulegium aerial parts during phenological stages.|
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Relative contribution of stage development on flavonoids and proanthocyanidins showed approximately the same classification as polyphenols. In fact, the maximum of flavonoid and proanthocyanidin levels were detected during the full flowering stage with respectively 164.28 mg QE/g DW and 4.80 mg QE/g DW [Table 1].
3.3. Phenolic coumpounds profile
In order to elucidate metabolic variations of phenols at ST2 stage of M. pulegium, the profile of individual phenolic compounds was investigated and presented in [Table 2]. Total phenols identified were (12.94 ± 0.18) mg/g DW. Apigenin was the main flavone, with value reaching up to 6.01 mg/g DW of the total phenols. Diosmin (1.92 mg/g DW), p-coumaric acid (1.60 mg/g DW) and rosmarinic acid (1.66 mg/g DW) were also considered as major phenolics of pennyroyal extracts.
|Table 2: Contents of individual phenolic compounds (mg/g DW) of M. pulegium aerial parts during flowering stage (by elution order).|
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Identified phenolic compounds were classified into five chemical classes with values varying from 0.31 to 8.12 mg/g DW. M. pulegium extracts were rich in flavones [(8.12 ± 0.06) mg/g DW] and hydroxycinnamic acids [(3.26 ± 0.05) mg/g DW] which constituted the major classes during ST2 stage [Table 2]. However, the percentages of other classes did not exceed [(0.57 ± 0.02) mg/g DW for flavanols, (0.68 ± 0.04) mg/g DW for flavonols and (0.31 ± 0.01) mg/g DW for flavanones].
3.4. Phenological effect on antioxidant capacity
In vitro antioxidant activity of pennyroyal extracts was investigated during its different vegetable stages using two different and complementary methods (DPPH and reducing power tests). Interestingly, all M. pulegium extracts showed a scavenging ability on DPPH higher than that of commercial standard, BHT (IC50 = 7.5 μ g/mL) [Table 3].
|Table 3: Antioxidant properties against DPPH radical (IC50) and reducing power (EC50) of M. pulegium aerial parts (μ g/mL).|
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Concerning the effect of phenological stage, changes in the antioxidant activities of various plant extracts were of great importance from the nutritional and pharmaceutical points of view. In this regard, radical scavenging activity of pennyroyal methanolic extracts showed an increasing ability to scavenge the DPPH radicals from ST1 (IC50 = 0.80 μ g/mL) to ST3 (IC50 = 0.09 μ g/mL). Of the same, despite the moderate antioxidant activity by comparison to ascorbic acid [(42.00 ± 0.05) μ g/mL], lowest value of EC50 was recorded at ST3 reflecting the maximum of reducing power activity during this period (EC50 = 700 μ g/mL) [Table 3].
3.5. Relationship between total phenols and antioxidant activity
Correlation coefficients for DPPH, reducing power, total polyphenol, flavonoid and proanthocyanidin contents were given in [Figure 1] and [Figure 2]. According to these results, the antioxidant activity (in terms of free-radical scavenging activity and reducing power) did not show correlation with phenol contents.
|Figure 1: Correlations between free radical scavenging activity (test DPPH) and total polyphenol (a), flavonoid (b) and proanthocyanidin (c)contents.|
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|Figure 2: Correlations between reducing power activity and total polyphenol (a), flavonoid (b) and proanthocyanidin (c) contents.|
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3.6. Phenological effect on insecticidal activity
As demonstrated in [Table 4] and [Table 5], the repellent potentials of pennyroyal methanolic extracts during phenological stages were evaluated. For this, three dilutions (1/2, 1/4 and 1/8) from the solution stock of 1 mg/mL were tested against T. castaneum and L. serricorne.
|Table 4: Percentage repellency (%) of M. pulegium methanolic extracts against T. castaneum adults during phenological stages.|
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|Table 5: Percentage repellency (%) of M. pulegium methanolic extracts against L. serricorne adults during phenological stages.|
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Pennyroyal extracts showed strong repellent efficacy against the two coleopteran pests used. At a given exposure time, the repellent effect was significant and proportional to the concentration of the extract. Indeed, the repelling effect of the lowest dilution (0.125 mg/mL) during ST1 stage against T. castaneum was 6.66% after 1 h of exposure and 53.33% after 24 h of exposure. Similarly, for the highest concentration (1 mg/mL), the percentage of repulsion increased from 20% after 1 h of exposure to 80% after 24 h of exposure [Table 4]. Meanwhile, at the same doses, pennyroyal extracts reached only 20% and 56.66% repellency after 24 h of exposure against L. serricorne for 0.125 and 1 mg/mL respectively [Table 5].
The insecticidal effect of M. pulegium extracts varied depending on the phenological stage for the two beetles. Indeed, for the highest concentration (1 mg/mL), the repellent effect against T. castaneum reached its maximum (90%) during ST3 stage (after 24 h of exposure). However, this repellent effect was 80% during ST1 stage [Table 4]. As for L. serricorne, the same trend was underlined where insecticidal potential was less pronounced at ST2 and ST3 stages compared to ST1 stage. In fact, during ST2 and ST3 stages, the repulsion percentages increased from 10% to 30% and 43.33%, respectively, for the highest concentration (1 mg/mL) after 24 h of exposure [Table 5].
As mentioned in [Table 4] and [Table 5], we classified the level of repellency of M. pulegium extracts into six categories. During ST1 stage, pennyroyal extracts showed class IV repellency status for 1 mg/mL, 0.5 mg/mL and 0.25 mg/mL doses (against T. castaneum) after 24 h of exposure. However, this extract showed classes III, II and I respectively during ST1 stage (against L. serricorne) for the same doses.
Probit analysis showed that results of [Table 6] were also in line with those of [Table 4] and [Table 5]. The insecticidal activity of the two beetles was more pronounced during ST1 compared to ST2 and ST3 stages with the lowest repulsive doses ranging from 0.006 to 0.124 mg/mL for RD50 and from 3.027 to 13.902 mg/mL for RD95 respectively against T. castaneum and L. serricorne. Furthermore, RD50 and RD95 values of M. pulegium extracts tested with L. serricorne were higher than those of T. castaneum. Hence, we can note that L. serricorne was more susceptible to pennyroyal extract than T. castaneum adults.
|Table 6: Repellency doses RD50 and RD95 values of M. pulegium extracts against T. castaneum and L. serricorne adults after 24 h of exposure.|
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| 4. Discussion|| |
Interestingly, the Soxhlet extraction of pennyroyal aerial parts showed yield values better than those reported in the literature. Indeed, the study of Khadraoui et al showed yields of 1.54%, 1.62% and 4.77% (w/w, calculated on dry matter) of M. pulegium leaves at ST2 stage. This extraction wad made by three different solvents which were respectively; n-butanol, ethyl acetate and petroleum ether. Thus, this difference could be explained by the fact that petroleum ether, ethyl acetate and n-butanol are non-polar solvents and they are used to degrease the drugs and to extract lipophilic compounds (lipids, fatty acids, chlorophylls, etc.). In addition, several other studies have shown that methanol could be the best solvent capable of providing the higher extraction yield, which explains our choice in the present work.
However, our results showed lower values than those found by Sarikurkcu et al. Indeed, at the flowering stage, the yield of the methanolic extract of M. pulegium, collected from Turkey, reached up 22.85%. This difference may be due to the seasonal and regional variations characterizing the two collecting regions of pennyroyal. The impact of other factors on the extraction yield could be reported in many other studies such as the type of solvent used and its coefficient of diffusion, the particle size and the extraction method.
Concerning phenolic contents, our findings were in agreement with those reported by Karray-Bouraoui et al where phenolic composition of M. pulegium shoots from Tunisian (Bouarada) showed a polyphenol amount of 25 mg GAE/g DW at full flowering stage. On the other hand, a recent study lead by Sarikurkcu et al focused on the composition of polyphenol contents and the antioxidant activity of pennyroyal aerial parts from Turkey which demonstrated lower polyphenol content (97.20 μ g GAE/g DW). So, this variation may be due to the collection region effects. Obtained results highlighted the richness of pennyroyal aerial parts in total flavonoids compared to other researches such as Sarikurkcu et al and Khennouf et al who determined levels of 20.88 μ g QE/g DW and 35.16 μ g QE/g DW respectively of M. pulegium grown in Turkey and Algeria. These values were significantly lower than those found in this study, suggesting geographic variations related to climatic and/ or soil factors. As for proanthocyanidins, many other factors may affect these secondary metabolite productions such as intraspecific variations. Thereby, there is no general trend for phenolics distribution depending on genotype and harvest time. However, the majority of mint species showed that the biosynthesis of the highest content of phenolic compounds was enhanced in the stage of full flowering, mainly during the time of ultraviolet radiation,.
In agreement to our observations, several researchers have reported an increase in phenolic concentrations among different Lamiaceae species such as sweet marjoram (Origanum majorana L.), Lavandin (Lavandula x intermedia) and Melissa officinalis. Hence, according to Herms and Mattson, the concentrations of some secondary metabolites (particularly low molecular-weight phenolics) are synthesized only during early stages of seedling growth.
In terms of compositional analysis, HPLC analysis of pennyroyal extracts identified 11 phenolics owned to five chemical classes. These findings were in line with the recent study of Aires et al where Apigenin was found as the major phenolic compound in M. pulegium from Portugal. While differing for other mint species, rosmarinic and chlorogenic acids were found as the major identified compounds respectively in Mentha spicata and Mentha rotundifolia leaves collected from Algeria. These differences could be related to genetic, intraspecific factors and agroclimatic conditions. For instance, apigenin is considered as a potential health promoting and functional food agent thanks to its numerous biological activities such as cytostatic and cytotoxic effects. Of the same, this flavone is known as antioxidant, anti-inflammatory, anti-mutagenic, and anti- tumorigenic agent. Therefore, the presence of high levels of this compound in pennyroyal makes this plant very promising as natural source of bioactive principles.
This may explain the high antioxidant and anti-inflammatory potentials during this period. So, full flowering stage was determined as an ideal time to harvest the utmost contents of phenols from Tunisian pennyroyal.
The antioxidant activity of pennyroyal extracts was evaluated using DPPH test and reducing power ability. Results of our study showed that the antiradical activity of methanolic extracts was better than many other Mentha species reported in the litterature such as; Mentha spicata (IC50 = 87.89 μ g/mL), Mentha rotundifolia (IC50 = 21.71 /*g/mL), Mentha longifolia (IC50 = 24.07 μ g/mL) and Mentha piperita (IC50 = 13.32 μ g/mL). In another hand, these extracts showed moderate ability to reduce the iron ions by comparison to ascorbic acid (EC50 = 42 μ g/mL). In this context, the researches of Sarikurkcu et al and Khaled-Khodja et al have reported a reducing power of M. pulegium lower than the present work with respectively EC50 of 1.224 mg/mL (from Turkey) and (427.5 ± 15.41) mg/mL (from Algeria). This variation may be explained by the extraction solvent effect, plant organ (leaves, fruit, bark, etc.) and geographical factors as well as the method used to determine the antioxidant activity. Moreover, this strong activity of M. pulegium could be mainly attributed to its richness in flavonoids (as described above). In fact, it is believed that these compounds may act as potent free radical scavengers and reducing agents.
It should be noted that to the best of our knowledge, no report was undertaken on the antioxidant activity of pennyroyal aerial parts during phenological stages. However, investigations about other plants were reported. Indeed, as agreed with our results, Gruľová et al showed that the highest antioxidant activity of peppermint (Mentha piperita, Lamiaceae) leaves collected in Slovakia was determined at the end of growing season period i.e. August–September (during fructification) (with IC50 between 56.29–57.35 μ g/mL). This increase may be due to climatic factors, mainly the low precipitation (12.4-24.8 mm) and the high temperatures. Meanwhile, a study conducted by Wang et al, showed an inverse trend compared with our findings. The antioxidant capacity (measured by FRAP assay) of jujube (Ziziphus jujuba Miller) fruits decreased during three edible maturity stages.
Statistical analysis with an objective to find any correlation between phenolic ontents and their antioxidant potential showed that the antioxidant activity does not only depend on the content of phenolic compounds but also on their nature (number of available hydroxyl groups and complexity of reaction kinetics) and the interactions between them.
In fact, according to several researchers, total extracts are complex mixtures of many different compounds which may be without a phenolic structure and distinct activities.
In contrast to our results, other reports showed that phenolic content could be used as an indicator of antioxidant properties of many plants. In fact, a positive correlation between polyphenols extracted from Sidastrum micranthum and Wissadula periplocifolia crude extracts and their antioxidant abilities was shown by De Oliveira et al. In addition, a high correlation was found between the DPPH scavenging potency and total phenolic contents of traditional plant species such as Allium sativum and Pistacia lentiscus.
Several studies have demonstrated the risk of acute and chronic effects for farmers and consumers exposed to pesticides and their harmful side effects including respiratory, cutaneous, neurological and reproductive problems. For this reason, researchers are looking for other natural alternatives of plant origin. In this study, insecticidal effect of M. pulegium was investigated and showed interesting dose and time-dependent repellency effect against the two beetles; T. castaneum and L. serricorne.
As compared to Mentha essential oil, there is not any publication focusing on the repellent potential of M. pulegium aerial parts extracts against coleopteran insects. Nevertheless, insecticidal activities of other mint species extracts against different pests were reported by many previous studies. In fact, Mangalat et al have reported the efficacy of Mentha rotundofolia leaves extracts against the mosquito Culex pipiens (30% mortality) after 48 h of exposure. On a related field study, Pascual-Villalobos and Robledo obtained a 0.05% repellency effect of Mentha longifolia hexane extract (after 2 h of exposure) against T. castaneum. While, Regnault-Roger and Hamraoui used dry material of Mentha piperita and obtained a mortality of 47.7% against Acanthoscelides obtectu. Hence, as compared to these species, M. pulegium could be considered as the most interesting natural insecticide. Likewise, its effectiveness could be related to its chemical composition.
In another hand, the repellency potential of plant extracts depends upon the extraction solvent. In fact, Pascual-Villalobos and Robledo have found that solvent polarity has a positive impact on the extraction of bioactive components responsible for the insecticidal effect. Similarly, extraction process, the age of the targeted pest organism and the developmental stage of the plant used were among the factors that may influence the insecticidal effect,,.
In the light of these results, this study showed a good potential of the use of M. pulegium as natural repellent agent against Tribolium and Lasioderma instead to synthetic pesticides. However, all these applications need further analyses with the intention of understanding the access mode of bioactive molecules responsible of the repellent activity of pennyroyal extract.
In conclusion, the determination of optimum conditions for phenol accumulation as well as their biological activities is an important field in order to valorize M. pulegium as source of natural phytochemicals. As a whole, our findings showed that polyphenol, flavonoid and proanthocyanidin contents and their antioxidant capacities depend on the plant stage of this medicinal plant. The fructification stage was considered as a proper harvesting period for M. pulegium to have high antioxidant potentials. Of the same, the results highlighted the strong repellent capacity of M. pulegium extracts especially during early vegetable stage. Therefore, this source of natural products could be considered as a promising tool to replace synthetic insecticides. However an intensive research on the mechanism of insect action, a study of synergistic effects between different constituents of pennyroyal extract and the search of new additives are the important items to improve commercial and food repellent formulations.
Conflict of interest statement
The authors declare that there is no conflict of interest.
This topic was undertaken by a Project of Valorization of the Results of Research (VRR) and funded by the Ministry of Higher Education and Scientific Research in Tunisia.
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[Figure 1], [Figure 2]
[Table 1], [Table 2], [Table 3], [Table 4], [Table 5], [Table 6]