|Year : 2018 | Volume
| Issue : 4 | Page : 189-193
Comparison of antioxidant capacity and α -glucosidase inhibitory activity between bitter melon (Momordica charanti) fruit and leaf extract
Department of Nutrition and Culinary Science, Hankyong National University, 327 Chungang-ro, Anseong-si, Gyeonggi-do, 17579, Korea
|Date of Submission||03-Feb-2018|
|Date of Decision||14-Mar-2018|
|Date of Acceptance||16-Apr-2018|
|Date of Web Publication||30-Apr-2018|
Department of Nutrition and Culinary Science, Hankyong National University, Gyeonggi-do
Source of Support: None, Conflict of Interest: None
Objective: To compare the physiologically active substances, antioxidant and antidiabetic activities in vitro of bitter melon fruit and leaf extract. Methods: Total polyphenol and flavonoid contents were measured using spectrophotometrically by gallic acid and catechin standard curves, respectively. The radical-scavenging potential of bitter melon fruit and leaf extract were measured by DPPH, ABTS and hydroxyl radical scavenging ability and reducing power and anti-diabetic ability was evaluated by α -glucosidase activity. Results: It was confirmed that the bitter melon leaf contained more total polyphenols and flavonoids than bitter melon fruits. Bitter melon leaf extract contained 2.8-fold and 4.9-fold higher total polyphenols and flavonoids than bitter melon fruits, respectively. The DPPH radical scavenging activity of bitter melon leaf was 5.81- and 5.70-fold higher than that of the bitter melon frui, based on 200 μg/mL and 400 μg/mL of the extract, respectively. In ABTS, hydroxyl radical scavenging ability and reducing power, the bitter melon leaf extract all showed higher antioxidant capacity than the bitter melon fruit. Bitter melon fruit showed 2.52- and 2.63-fold higher α -glucosidase inhibitory activity than bitter melon leaf extract at 200 μg/mL and 400 μg/mL, respectively. Conclusions: Based on our results, bitter melon may improve antidiabetic effects by upreguating α -glycosidase activity. Even, bitter melon leaf extract shows higher antioxidant potential than its fruit but bitter melon leaf extract does not show higher α -glucosidase inhibitory potential than bitter melon fruit. The overall results support that bitter melon fruit and leaf may have specific target effects on antidiabetic and antioxidant, respectively.
Keywords: Bitter melon, Polyphenols, Flavonoids, Antioxidant, Antidiabetic
|How to cite this article:|
Hwang ES. Comparison of antioxidant capacity and α -glucosidase inhibitory activity between bitter melon (Momordica charanti) fruit and leaf extract. Asian Pac J Trop Biomed 2018;8:189-93
|How to cite this URL:|
Hwang ES. Comparison of antioxidant capacity and α -glucosidase inhibitory activity between bitter melon (Momordica charanti) fruit and leaf extract. Asian Pac J Trop Biomed [serial online] 2018 [cited 2019 Jul 15];8:189-93. Available from: http://www.apjtb.org/text.asp?2018/8/4/189/231280
Foundation project: This study was funded by the Basic Science Research Program supported by the National Research Foundation of Korea (NRF-2016R1A2B4014977).
| 1. Introduction|| |
Bitter melon (Momordica charantia) is cultivated in Middle East, Africa, India and China as a tropical or semi-tropical plant beloinging to the Cucurbitaceae family. It is apparently similar to cucumber, immature fruit is emerald green and orange-yellow color changes with maturity. It has a very bitter taste and is consumed as a vegetable in an immature state, while it is used as condiments when it is mature. Bitter melon has been widely used as a natural or folk remedy to treat diabetes by the general public. It has also been used to alleviate symptoms of kidney-stone, piles, galactagogue, contraceptive, abortifacient, anthelminitic, gout, and pneumonia,,,.
Bitter melon has large amoutns of physiologically active substances and various vitamins. It is rich in minerals, such as zinc, iron, magnesium, calcium and is an excellent source of fiber. Especially, bitter melon contains a special triterpenoid called charantin, which has been reported to have excellent antidiabetic properties. Bitter melon has high amount of momorcharins, momordins. These compounds inactivate ribosomes, and momordins alter gastrointestinal traisit time and lower the blood glucose,.
Traditionally, the wild bitter leaves have been used to treat burns, insect bites, rashes, bee strings by juicing. It is reported that drinking tea with boiled bitter melon leaves and its fruits are good for preventing or treating hypertentsion, betes, stomachache, toothache, liver diseases.
Fruit leaves are agricultural by-product of the tree, and contain more antioxidants of natural source, such as polyphenols, flavonoids, lignin and semi-secoirdoids than those of fruits,. Previous studies have indicated that fruit leaves such as blackcurrent, bilberry, sea buckthorn and quince leaves exhibit numerous health-promoting biological activites inculidng antioxidant activity, lipid peroxidation inhibition and anti-mutation activitie,,.
At present, several studies have been reported on antioxidant activity of bitter melon fruit and there is little information available on bitter melon leaf. The knowledge about active compounds of bitter melon leaf are mostly unknown. This study was conducted to investigate the physiologically active substances in bitter melon fruit and leaf extract, and to measure their antioxidant and antidiabetic activities in vitro.
| 2. Material and methods|| |
Gallic acid, catechin, Folin-Ciocalteu's phenol reagent, 2,2-diphenyl-1-picrylhydrazyl radical (DPPH), 2,2'-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS), nitro blue tetrazolium chloride, nicotinamide adenine dinucleotide, Tris-HCl, paramethyl styrene, sodium phosphate monobasic, and α -glucosidase were purchased from Sigma Chemical (Sigma-Aldrich Co., St. Louis, MO, USA). Hydrogen peroxide, trichloroacetic acid, ethylendiaminetetraacetic acid, and sodium phosphate dibasic anhydrous were purchased from SamChun Chemical (Pyeongtaek city, Korea). 2-thiobarbituric acid was purchased from Wako chemical (Tokyo, Japan) and all chemicals used were of analytical grade.
2.2. Sample preparation
Bittere melon fruit and leaf grown in Cheonan, Korea in October 2016 were purchased. Water-washed bitter melon fruit was cut to a thickness of 0.5 mm and freeze-dried in a freeze-drier (Ilshin BioBase, Dongducheon, Korea) after freezing at -80 °C. Bitter melon leaf was also washed and freeze-dried after freezing at -80 °C. The lyophilized bitter melon fruit and leaf were finely ground using a blender (Tefal, Seoul, Korea). Lyophilized powder sample (5 g) was taken in an Erlenmeyer flask, then 125 mL of 80% ethanol was added and extracted for 2 h at 60 °C.
The extracts were filtered on a vacuum connected device using Whatman #2 filter paper (Whatman International Ltd., Maidstone, UK), and this extraction procedure was repeated 3 times. The filtered liquid was evaporated using a rotary vacuum concentrator (EYELA, Tokyo, Japan) at 40 °C. The concentrated extract was dried with a freeze-drier and pulverized so that the particle sizs was less than 1.0 mm. The samples were air-sealed, placed in a plastic bag and stored at -20 °C for the next experiment.
2.3. Measurement of total polyphenol and flavonoid contents
Total polyphenol contents were determined by referring to the color measurement method of Folin-Ciocalteu. The total flavonoid content in the samples was determined with reference to Woisky and Salatino.
2.4. Measurment of antioxidant capacity
The DPPH radical scavenging ability of the sample was measured with the method of Cheung et al. The ABTS radical scavenging ability of the sample was measured by Re et al. The hydroxyl scavenging ability of the sample was measured by Chung et al. The reducing power of the sample was measured by Oyaizu.
2.5. Measurement of α -glucosidase activity
The α -glucosidase activity of the sample was measured by reference to the method of Kim et al. α -Glucosidase was dissolved in 100 mM sodium phosphate buffer (pH 7.0) at a concentration of 0.2 unit/mL. A total of 2 μL of the sample and 100 μL of the enzyme solution were mixed well. Mixed samples were transferred to 96-well plate and absorbance at 405 nm was measured. Then, 2 mM p-nitrophenyl α -D-glucopyranoside (100 μL) was rapidly added into 96-well plate and the absorbance at 405 nm was further measured. Acabose (100 μg/mL) which was used to proven the α -glucosidase inhibitory activity was used as a positive control, and the α -glucosidase activity was calculated according to the following formula.
Inhibitory activity (%) = (Absorbanesample / Absorbancecontrol) x100
2.6. Statistical analysis
Statistical analysis was performed using the SPSS software package, version 17.0 (Statistical Package for the Social Sciences,
Spss Inc., Chicago, IL, USA), and the significance was verified by one-way analysis of variance. P<0.05 was considered significant.
| 3. Results|| |
3.1. Total polyphenol and flavonoid contents
The content of total polyphenol measured by gallic acid equivalent was (16.15±0.31) mg/g in bitter melon fruit and (45.55±0.34) mg/g in bitter melon leaf. The total flavonoid content was measured on the basis of catechin, (9.70±0.27) mg/g in bitter melon fruit and (47.25±1.48) mg/g in bitter melon leaf. According to the results, total polyphenol and flavonoid contents were higher in bitter melon leaf extract than bitter melon fruit extract.
3.2. Antioxidant activity
Antioxidant activities of bitter melon fruit and leaf extract were measured by in vitro method for inhibtion of DPPH, ABTS and hydroxyl radical formation and reducing power. The DPPH radical-scavenging ability depends on the concentration (25–400 μg/mL) of bitter melon fruit and leaf extract, and the higher the extract concentration, the higher the DPPH redical formation was suppressed to a higher percentage [Table 1]. The 100 μg/mL ethanol extract of bitter melon fruit inhibited the production of DPPH radicals by 2.50% on average, and the DPPH radical production was suppressed up to 5.38% at 200 μg/mL and 10.63% at 400 μg/mL of bitter melon fruit extract. The inhibition of DPPH radical formation at 100 μg/mL of ethanol extract of bitter melon leaf was 16.23%, and the in inhibition rate was increased to 31.28% and 60.63% as the concentration of extract increased to 200 μg/mL and 400 μg/ mL, respectively. The DPPH radical scavenging activity of the bitter melon leaf extract was 5.81- and 5.70 times higher than that of bitter melon fruit at 200 μg/mL and 400 μg/mL, respectively.
|Table 1: DPPH radical-scavenging activity (%) of bitter melon fruit and leaf (mean ± SD).|
Click here to view
As shown in [Table 2], as the concentration of bitter melon fruit and leaf extract increased from 25 μg/mL to 400 μg/mL, the ABTS radical scavenging ability was also increased. The bitter melon fruit extract inhibited ABTS radical formation up to 3.45% at 100 μg/mL, 6.38% at 200 μg/mL and 11.75% at 400 μg/mL. In the case of bitter melon leaf extract, inhibition of ABTS radical formation was 10.01% at 100 μg/mL, 20.28% at 200 μg/mL, and 45.30% at 400 mL, which showed a higher inhibition rate than the fruit extract of bitter melon. The extract of bitter melon leaf showed ABTS radical scavenging activities of 3.24- and 3.86 times higher than those of bitter melon fruit at 200 μg/mL and 400 μg/mL, respectively.
|Table 2: ABTS radical-scavenging activity (%) of bitter melon fruit and leaf (mean ±SD).|
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When compared based on the same concentration, the bitter melon leaf extract had a higher hydroxyl radical-scavenging power than the bitter melon fruit [Table 3]. At the same concentration, the extracts of bitter melon leaf showed stronger hydroxyl radicals-scavenging activity at 400 μg/mL than those of bitter melon fruit at the same concentration. The inhibition of hydroxyl radical formation at 100 μg/mL of ethanol extract of bitter melon leaf was also higher than that of bitter melon fruit. The ethanol extract of bitter melon leaves showed stronger radical-scavenging power than bitter melon fruit. Bitter melon leaf extract showed 1.48- and 1.30 times higher hydroxyl radical scavenging power at 100 μg/mL and 400 μg/mL, respectively, than the bitter melon fruit.
|Table 3: Hydroxyl radical-scavenging activity (%) of bitter melon fruit and leaf (mean ± SD).|
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The reducing power of ethanol extracts in bitter melon fruit and leaf is shown in [Table 4], and numerically higher absorbance means that the reducing power is high. The absorbance values of bitter melon fruit and leaf extracts were 0.19 and 0.23 at 100 μg/mL, respectively. The highest absorbance was observed in bitter melon leaf extracts at 400 μg/mL (OD700 nm 0.43) which is 1.48-fold higher reducing power than bitter melon fruit extract at the same concentration.
|Table 4: Reducing power activity (absorbance at 700 nm) of bitter melon fruit and leaf (mean ± SD).|
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3.3. Determination of α -glucosidase activity
[Table 5] shows that the higher the concentration of bitter melon fruit and leaf extracts, the greater the inhibitory activity of α -glucosidase. The α -glucosidase inhibitory effect of bitter melon fruit was higher than its leaf extracts at the same concentration. The average α -glucosidase inhibitory activity at 50 μg/mL in bitter melon fruit was 23.97%, but lowered in leaf extracts as -5.13%. At 100 μg/mL of bitter melon extract, α -glucosidase inhibitory activity of bitter melon fruit was 30.00, which was higher than those in the bitter melon leaf extracts and acarbose, 2.59% and 22.66%, respectively. Bitter melon fruit showed 2.52- and 2.63-fold higher α -glucosidase inhibitory activity than bitter melon leaf extracts at 200 μg/mL and 400 μg/mL, respectively. Bitter melon fruit showed better inhibitory effects of α -glucosidase than acarbose which is commercially used antidiabetic drug to treat diabetes mellitus type 2 in some countries. Based on our results, bitter melon fruit may improves antidiabetic effects by upreguating α -glycosidase activity. Even, bitter melon leaf extracts showed higher antioxidant potential than its fruit but bitter melon leaf extracts did not show α -glucosidase inhibitory potential than bitter melon fruit.
|Table 5: α -Glucosidase inhibitory activity of bitter melon fruit and leaf (%) (mean ±SD).|
Click here to view
| 4. Discussion|| |
The bitter melon contains various substances such as catechin flavonoids, caffeic acid, p-coumarc acid, ferrulic acid, isoflavones, terpenes, glucosinolates, and these substances cause bitter taste,,. Teleszko and Wojdylo have determined the polyphenol compounds from the 7 selected edible fruits (apple, quince, Japanese quince, chokeberry, blackcurrant, cranberry and bilberry) and their leaves. They found that fruit leaves contained higher amount of polyphenol compounds than those of fruits. According to the study, the highest content was in the order of quice leaves, cranberry, apple, chokeberry, Japanese quince, bilberry, and blackcurrant. In the leaves of cranberry, the difference was 20-fold, in the case of Japanese quince 23-fold, in bilberry and quince 13-fold, in blackcurrant 9-fold, and 7-fold in the case of chokeberry leaves and fruits.
The antioxidant activity of bitter melon leaf extracts was higher than that of fruit. Bitter melon fruit showed 2.52- and 2.63-fold higher α -glucosidase inhibitory activity than bitter melon leaf extracts at 200 μg/mL and 400 μg/mL, respectively. Bitter melon leaf extract showed higher antioxidant potential than its fruit but did not show α -glucosidase inhibitory potential compared to its fruit. Overall, the results of this experiment indicate that bitter melon fruits have an effect on diabetes and bitter melon leaves are effective in preventing or delaying oxidation.
Accorind to the results of Wu and Ng, the inhibitory activity of bitter melon on DPPH radical formation was highe in water extracts than in ethanol extracts, suggesting that water-soluble oxidation inhibitors were dissolved in water extract. Teleszko and Wojdylo[l2] reported that antioxidant activity of leaves were higher than their fruits. The strongest antioxidant potential determined by ABTS assay was observed by qunce leaves and these values were 15- and 12-fold higher compared to the fruits. The higher content of total polyphenols and flavonoids in lentil leaves is known to have the ability to inhibit the formation of DPPH and ABTS radicals.
α-Glucosidase (EC 126.96.36.199), which is distributed in small intestinal epithelial cells, play a role in liberating glucose molecules by decomposing 1,4-α -glucopyranoside bonds in carbohydrates. Thus, α -glucosidase plays an important role in regulating blood glucose levels after meals and keeping blood glucose levels within normal ranges. The inhibition of α -glucosidase may delay the hydrolysis of carbohydrates and induce glucose uptake in the small intestine. Therefore, the substance that inhibits the secretion of α -glucosidase can be used as a treatment for type 2 diabetes, which has the highest blood glucose level after ingesting carbohydrates, and can inhibit the digestion of carbohydrates including starch and table sugar in the small intestine. In general, carbohydrates are converted to simple sugars and absorbed from the intestine. Thus, α -glucosidase inhbitors reduce blood sugar by inhibiting the conversion of carbohydrates to simple sugar. Matsuura et al reported that administration of bitter melon juice after inducing diabetes by injecting streptozotocin into rats suppressed α -glucosidase activity and decreased blood glucose levels. Several studies have reported that blood glucose levels are reduced by injecting bitter melon juice or whole powder into people with type 2 diabetes or diabetes-induced rodents,,.
Conflict of interest statement
We declare that we have no conflict of interest.
This study was funded by the Basic Science Research Program supported by the National Research Foundation of Korea (NRF-2016R1A2B4014977).
| References|| |
Kenny O, Smyth TJ, Hewage CM, Brunton NP. Antioxidant properties and quantitative UPLC-MS analysis of phenolic compounds from extracts of fenugreek (Trigonella foenum-graecum)
seeds and bitter melon (Momordica charantia)
fruit. Food Chem
Begum S, Ahmed M, Siddiqui BS, Khan A, Saify ZS, Arif M. Triterpenes, a sterol and a monocyclic alcohol from Momordica charantia. Phytochem
Grover JK, Yadav SP. Pharmacological actions and potential uses of Momordica charantia:
A review. J Ethnopharmacol
Leung L, Birwhistle R, Kotecha J, Hannah S, Cuthberson S. Anti-diabetic and hypoglycaemic effects of Momordica charantia
(bitter melon): A mini review. Br J Nutr
Joseph B, Jini D. Antidiabetic effects of Momordica charantia
(bitter melon) and its medicinal potency. Asian Pac J Trop Dis
Bakare RI, Magbagbeola OA, Akinwande AI, Okunowo OW. Nutritional and chemical evaluation of Momordica charantia. J Med Plant Res
Krawinkel MB, Keding GB. Bitter gourd (Momordica charantia):
A dietary approach to hyperglycemia. Nutr Rev 2006; 64
Chao CY, Huang CJ. Bitter gourd (Momordica charantia)
extract activates peroxisome proliferators-activated receptors and upregulates the expression of the acyl-CoA oxidase gene in H4IIEC3 hepatoma cells. J Biomed Sci
Wu SJ, Ng LT. Antioxidant and free radical scavenging activities of wild bitter melon (Momordica charantia
Linn. Var. abbreviate
Ser.) in Taiwan. LWT-Food Sci Technol
Chiu NY, Chang KH. The illustrated medicinal plants of Taiwan
. Vol. 4. Taipei, Taiwan: SMC Publishing Inc; 1995, p. 213.
Caturla N, Pérez-Fons L, Estepa A, Micol V. Differential effects of oleuropein, a biophenol from Olea europaea
, on anionic and zwiterionic phospholipid model membranes. Chem Physic Lipid
Teleszko M, Wojdylo A. Comparison of phenolic compounds and antioxidant potential between selected edible fruits and their leaves. J Func Food
Chen T, Kang Z, Yen M, Huang M, Wang B. Inhibitory effect of aqueous extracts from miracle fruit leaves on mutation and oxidative damage. Food Chem
Chen GL, Zhang X, Chen SG, Han MD, Gao YQ. Antioxidant activities and contents of free, esterified and insoluble-bound phenolics in 14 subtropical fruit leaves collected from the south of China. J Func Food
Zhou K, Su L, Yu L. Phytochemicals and antioxidant properties in wheat bran. J Agric Food Chem
Woisky R, Salatino A. Analysis of propolis: Some parameters and procedures for chemical quality control. J Apicul Res
Cheung LM, Cheung PC, Ooi VE. Antioxidant activity and total phenolics of edible mushroom extracts. Food Chem
Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Rad Biol Med
Chung SK, Osawa T, Kawakishi S. Hydroxyl radical-scavenging effects of spices and scavengers from brown mustard (Brassica nigra). Biosci Biotechnol Biochem
Oyaizu M. Studies on product of browning reaction prepared from glucose amine. Jap J Nutr
Kim MA, Son HU, Yoon EK, Choi YH, Lee SH. Comparison of antidiabetic activities by extracts of grape cultivar. Korean J Food Preser
Raina K, Kumar D, Agarwal R. Promise of bitter melon (Momordica charantia)
bioactives in cancer prevention and therapy. Seminar Cancer Biol
Snee LS, Nerurkar VR, Dooley DA, Efird JT, Shovic AC, Nerurkar PV. Strategies to improve palatability and increase consumption intentions for Momordica charantia
(bitter melon): A vegetable commonly used for diabetes management. Nutr J
Flanagan PR, Forstner GG. Purification of rat intestinal maltase/glucoamylase and its anomalous dissociation either by heat or by low pH. Biochem J
S0rensen SH, Norén O, Sjöström H, Danielsen EM. Amphiphilic pig intestinal microvillus maltase/glucoamylase structure and specificity. Eur J Biochem
Matsuura H, Asakawa C, Kurimoto M, Mizuntani J. Alpha-glucosidase inhibitor from the seeds of balsam pear (Momordica charantia)
and the fruit bodies of Grifola frondosa. Biosci Biotechnol Biochem
Chen Q, Chan LL, Li ET. Bitter melon (Momordica charantia)
reduces adiposity, lowers serum insulin and normalizes glucose tolerance in rats fed a high fat diet. J Nutr
Virdi J, Sivakami S, Shahani S, Suthar AC, Banavalikar MM, Biyani MK. Antihyperglycemic effects of three extracts from Momordica charantia. J Ethnopharmacol
Ahmed I, Adeghate E, Cummings E, Sharma AK, Singh J. Beneficial effects and mechanism of action of Momordica charantia
juice in the treatment of streptozotocin-induced diabetes mellitus in rat. Mol Cell Biochem
[Table 1], [Table 2], [Table 3], [Table 4], [Table 5]