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BASIC RESEARCH
Year : 2018  |  Volume : 8  |  Issue : 3  |  Page : 160-165

Antidiabetic potential of methanol extracts from leaves of Piper umbellatum L. and Persea americana Mill.


1 Laboratory of Microbiology and Antimicrobial Substances, Faculty of Science, University of Dschang, P.O. Box 67 Dschang, Cameroon; Centre for Advanced Drug Research, COMSATS Institute of Information Technology, Abbottabad-22060, Pakistan
2 Centre for Advanced Drug Research, COMSATS Institute of Information Technology, Abbottabad-22060, Pakistan
3 Laboratory of Microbiology and Antimicrobial Substances, Faculty of Science, University of Dschang, P.O. Box 67 Dschang, Cameroon
4 Institute of Fisheries and Aquatic Sciences, University of Douala, P.O Box 7236, Douala, Cameroon
5 Centre for Advanced Drug Research, COMSATS Institute of Information Technology, Abbottabad-22060, Pakistan; Department of Chemical Sciences, Afe Babalola University Ado, P.M.B 5454, Nigeria

Correspondence Address:
Guy Sedar Singor Njateng
Laboratory of Microbiology and Antimicrobial Substances, Faculty of Science, University of Dschang, P.O. Box 67 Dschang, Cameroon; Centre for Advanced Drug Research, COMSATS Institute of Information Technology, Abbottabad-22060, Pakistan

Jamshed Iqbal
Centre for Advanced Drug Research, COMSATS Institute of Information Technology, Abbottabad-22060
Pakistan
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/2221-1691.227997

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Objective: To determine inhibitory activity of methanolic leaf extract of Piper umbellatum and Persea americana (P. americana) (traditionally used in Cameroon against diabetes) on α -glucosidase, β -glucosidase, maltase-glucoamylase, aldose reductase and aldehyde reductase activities, enzymes involved in starch digestion or diabetic complications. Methods: The methanol extracts from Piper umbellatum and P. americana were prepared by maceration. To assess relative efficacy of these extracts, the determination of concentrations that were needed to inhibit 50% of enzyme activity was done, whereas, gas chromatography-mass spectrum was used to identify components from extracts that may be responsible for the activities. Results: The tested extracts strongly inhibited α -glucosidase, maltase-glucoamylase, aldose reductase and aldehyde reductase activities with IC50 ranging from (1.07 ± 0.03) to (31.77 ± 1.17) μg/mL. Among the tested extracts, P. americana was the most active against sensitive enzymes (IC50 of 1.07 ± 0.03 to 15.63 ± 1.23). But, none of the extracts showed interesting inhibitory effect against β -glucosidase as their percentage inhibitions were less than 16%. From gas chromatography-mass spectrum analysis, 10 and 8 compounds were identified in Piper umbellatum and P. americana extracts respectively, using NIST library 2014. Conclusions: Results of this study provide the scientific credential for a prospective usage of these plants to treat diabetes.


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