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Year : 2018  |  Volume : 8  |  Issue : 1  |  Page : 7-13

Effective Aeromonas specific monoclonal antibody for immunodiagnosis

1 Department of Microbiology and Immunology, Faculty of Tropical Medicine, Mahidol University, Thailand
2 Department of Microbiology, Faculty of Public Health, Mahidol University, Thailand
3 Department of Medical Technology, Faculty of Science and Technology, Bansomdejchaopraya Rajabhat University, Thailand

Correspondence Address:
Yuvadee Mahakunkijcharoen
Department of Microbiology and Immunology, Faculty of Tropical Medicine, Mahidol University, 420/6 Rajvithi Road, Ratchathewi, Bangkok 10400
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Source of Support: None, Conflict of Interest: None

DOI: 10.4103/2221-1691.221128

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Objective: To identify the monoclonal antibody specific to Aeromonas spp., a Gram negative bacteria causing gastroenteritis and wound infection. Methods: The monoclone, namely 88F2-3F4, was produced from hybridoma technology. The specificity of antibody secreted from 88F2-3F4 was tested against other Gram negative bacteria frequently found in gastrointestinal tract. Then the antibody was used for searching Aeromonas antigens in artificial seeded rectal swab cultures by dot-blot enzyme linked immunosorbent assay. Results: 88F2-3F4 produced an antibody that recognized an antigen with a molecular mass of 8.5 kDa in all 123 isolates of the seven Aeromonas species tested, but recognized no epitope of any other Gram-negative bacterium typically found in the gastrointestinal tract. A dot-blot enzyme linked immunosorbent assay based on this antibody showed 86.49% sensitivity and 92.13% specificity. Conclusions: 88F2-3F4 monoclonal antibody could react with all Aeromonas isolates, but not other Gram negative bacteria, therefore it should be a useful tool for the detection of Aeromonas antigen in clinical and environmental samples.

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